Isolation and characterization of a cold-resistant PCB209-degrading bacterial strain from river sediment and its application in bioremediation of contaminated soil
Isolation and characterization of a cold-resistant PCB209-degrading bacterial strain from river sediment and its application in bioremediation of contaminated soil
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DOI：
10.1080/10934529.2015.1094324
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
L. Qiu;Hu Wang;Xuntao Wang
中科院分区：
文献类型：
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作者：
L. Qiu;Hu Wang;Xuntao Wang
摘要从渭河底泥中分离到一株能降解2，2 ′，3，3 ′，4，4 ′，5，5 ′，6，6 ′-十氯联苯（PCB 209）的耐寒细菌（QL株）。经鉴定，QL菌株为革兰氏阴性杆菌，为睾丸酮丛毛单胞菌（Comamonastestosteroni）。C. testosteroni从未被报道能够在低温下降解多氯联苯209。在本研究中，降解特性表明菌株QL可以在低温（10 ± 0.5°C）下以PCB 209为唯一碳源生长。更重要的是，40%接种体积的菌株QL能够在140小时内完全降解PCB 209（PCB 209的初始浓度为100-500 µg L−1，10 ± 0.5°C和pH 7-8）。降解过程符合零级反应动力学。此外，实验室模拟和真实世界的现场实验表明，菌株QL是有效的，在实际应用中的PCB 209的生物降解污染土壤。
ABSTRACT A cold-resistant bacterium (strain QL) that can degrade 2,2′,3,3′,4,4′,5,5′,6,6′-decachlorobiphenyl (PCB209) was isolated from Wei-he River sediment. Strain QL was identified as a rod-shaped gram-negative bacterial strain, which was further identified as Comamonas testosteroni. C. testosteroni has never been reported to be capable of degrading PCB209 at low temperatures. In this study, the degradation characteristics showed that strain QL could grow with PCB209 as the sole carbon source at low temperatures (10 ± 0.5°C). More significantly, strain QL of 40% inoculation volume was able to completely degrade PCB209 in 140 h (initial concentration of PCB209 was 100–500 µg L−1 at 10 ± 0.5°C and pH 7–8). The degradation process proceeded with zero-order reaction kinetics. Moreover, both laboratory simulation and real-world field experiments demonstrated that strain QL was effective in practical applications of PCB209 biodegradation in contaminated soil.