Genetic assessment of the importance of galectin-3 in cancer initiation, progression, and dissemination in mice
Genetic assessment of the importance of galectin-3 in cancer initiation, progression, and dissemination in mice
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DOI：
10.1093/glycob/cwn105
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发表时间：
2009-01-01
期刊：
影响因子：
4.3
通讯作者：
Poirier, Francoise
中科院分区：
文献类型：
--
作者：
Eude-Le Parco, Isabelle;Gendronneau, Gaelle;Poirier, Francoise
β-半乳糖苷结合凝集素的半乳糖凝集素家族参与正常和病理过程。在许多癌症中已经描述了半乳糖凝集素-3的改变的表达，并且癌细胞系的研究已经暗示了这种凝集素在致瘤级联的各个方面。本报告的目的是通过将半乳糖凝集素-3无效突变（半乳糖凝集素-3（-/-））引入遗传编程为发展癌症的小鼠系中来直接评估半乳糖凝集素-3在肿瘤生物学中的重要性。我们使用两种人类肠癌小鼠模型，Apc（Min）和Apc（1638 N）系，研究肿瘤的发生和肿瘤的进展。我们还将半乳糖凝集素-3（-/-）小鼠与PyMT转基因动物杂交，这是一种原发性乳腺肿瘤以高频率引起肺转移的模型。出乎意料的是，我们发现，在这三种情况下，半乳糖凝集素-3的缺乏不会影响疾病的演变。
The galectin family of beta-galactoside binding lectins is involved in normal and pathological processes. Altered expression of galectin-3 has been described in many cancers, and studies of cancer cell lines have implicated this lectin in various aspects of the tumorigenic cascade. The goal of this report was to directly assess the importance of galectin-3 in tumor biology by introducing the galectin-3 null mutation (galectin-3(-/-)) into mouse lines genetically programmed to develop cancers. We used two mouse models of human intestinal cancer, the Apc(Min) and Apc(1638N) lines, to study tumor initiation and tumor progression. We also crossed the galectin-3(-/-) mice with PyMT transgenic animals, a model in which primary mammary gland tumors give rise to lung metastases at high frequency. Unexpectedly, we show that the absence of galectin-3 does not affect the evolution of the disease in any of these three situations.