Successful liver allografts in mice by combination with allogeneic bone marrow transplantation.
Successful liver allografts in mice by combination with allogeneic bone marrow transplantation.
复制标题
通过与同种异体骨髓移植相结合，在小鼠体内成功进行同种异体肝脏移植。
DOI：
10.1073/pnas.83.12.4529
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Ikehara,S
中科院分区：
文献类型：
--
作者：
Nakamura,T;Good,RA;Yasumizu,R;Inoue,S;Oo,MM;Hamashima,Y;Ikehara,S
通过与同种异体骨髓移植相结合，建立了成功的同种异体肝移植。当将 BALB/c (H-2d) 或 C57BL/6J (H-2b) 小鼠的肝组织切碎并移植到 C3H/HeN (H-2k) 小鼠的肾囊下时，会出现排斥反应。然而，当 C3H/HeN 小鼠接受 T 细胞耗尽的 BALB/c 或 BALB/c nu/nu 骨髓细胞或 BALB/c 小鼠胎儿肝细胞的照射和重建时，它们接受供体（干细胞）型（BALB/c）和宿主（胸腺）型（C3H/HeN）肝组织。混合淋巴细胞反应和细胞毒性 T 淋巴细胞诱导的测定表明，新开发的 T 细胞对供体（干细胞）型和宿主（胸腺）型主要组织相容性复合体决定簇具有耐受性。我们建议，对于肝硬化、肝癌等肝病患者，应考虑将同种异体肝移植联合骨髓移植作为一种可行的治疗方法。
Successful liver allografts were established by combination with allogeneic bone marrow transplantation. When liver tissue of BALB/c (H-2d) or C57BL/6J (H-2b) mice was minced and grafted under the kidney capsules of C3H/HeN (H-2k) mice, it was rejected. However, when C3H/HeN mice were irradiated and reconstituted with T-cell-depleted BALB/c or BALB/c nu/nu bone marrow cells, or with fetal liver cells of BALB/c mice, they accepted both donor (stem-cell)-type (BALB/c) and host (thymus)-type (C3H/HeN) liver tissue. Assays for both mixed-lymphocyte reaction and induction of cytotoxic T lymphocytes revealed that the newly developed T cells were tolerant of both donor (stem-cell)-type and host (thymus)-type major histocompatibility complex determinants. We propose that liver allografts combined with bone marrow transplantation should be considered as a viable therapy for patients with liver disease such as liver cirrhosis and hepatoma.