Localization of ATP-gated P2X receptor immunoreactivity in rat sensory and sympathetic ganglia
Localization of ATP-gated P2X receptor immunoreactivity in rat sensory and sympathetic ganglia
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DOI：
10.1016/s0304-3940(98)00774-5
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发表时间：
1998-11
影响因子：
2.5
通讯作者：
Z. Xiang;X. Bo;G. Burnstock
中科院分区：
文献类型：
--
作者：
Z. Xiang;X. Bo;G. Burnstock
采用免疫组织化学方法研究了atp门控阳离子通道家族P2X1、P2X2、P2X3、P2X4、p2x5和p2x6受体在大鼠三叉神经、背根、结节、颈上节和腹腔神经节中的分布。研究发现，针对6种P2X受体亚型的多克隆抗体可以标记这些神经节中90%以上的神经元的不同强度。每个神经节内单个神经元的免疫反应性强度也有相当大的差异。p2x3受体在背根和三叉神经节的表达明显高于其他5个亚型。p2x3免疫反应性在小直径神经元中明显强于在大直径神经元中。在结节神经节中，p2x2和p2x3抗体均能强烈标记神经元。在交感颈上神经节和腹腔神经节检测到5种P2X受体亚型（P2X5除外）的免疫反应性，其中P2X2、p2x4和p2x6表现出较高的强度。p2x3受体在交感神经节中的低水平表达表明该受体亚型并不局限于其高表达的感觉神经节。结果表明，感觉神经节和交感神经节均表达多种P2X受体亚型，并且不同亚型在不同神经元亚群中的表达水平不同。
The distribution of P2X1, P2X2, P2X3, P2X4, P2X5and P2X6receptors, a family of ATP-gated cation channels, in rat trigeminal, dorsal root, nodose, superior cervical, and coeliac ganglia was studied immunohistochemically. It was found that polyclonal antibodies for the six P2X receptor subtypes could label over 90% of neurones in these ganglia to different intensities. There was also considerable variation in intensity of immunoreactivity in individual neurones within each ganglion. In dorsal root and trigeminal ganglia expression of the P2X3receptor was much higher than for the other five subtypes. P2X3immunoreactivity was much more intense in the small-diameter neurones than in the large-diameter neurones. In nodose ganglion, both P2X2and P2X3antibodies labelled the neurones intensely. In sympathetic superior cervical and coeliac ganglia, immunoreactivity to five P2X receptor subtypes were detected (exception, P2X5), with P2X2, P2X4and P2X6showing higher intensity. Low level expression of P2X3receptor in sympathetic ganglia indicates that this receptor subtype is not limited to the sensory ganglia where it was highly expressed. The results have demonstrated that both sensory and sympathetic ganglia express a variety of P2X receptor subtypes and that different subtypes are expressed to different levels and by different subpopulations of neurones.