Clonally restricted anti-IgG antibodies in rheumatoid arthritis.
Clonally restricted anti-IgG antibodies in rheumatoid arthritis.
复制标题
类风湿性关节炎中克隆限制性抗 IgG 抗体。
DOI：
10.1002/art.1780271208
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发表时间：
1984
影响因子：
--
通讯作者：
Stevens,RH
中科院分区：
文献类型：
--
作者：
Persselin,JE;Louie,JS;Stevens,RH
通过等电聚焦（IEF）和色谱聚焦技术，在类风湿关节炎（RA）患者血清中检测到克隆限制性抗IgG抗体。抗Fab抗体主要是酸性蛋白质，等电点为4.5-6.5，并显示出有限的谱型模式。与IgG的Fc部分反应的蛋白质显示碱性pI为7.5-9.0的多克隆谱型模式。在6 Murea凝胶上通过IEF分析时，在RA血清中一致检测到有限的抗Fab谱型阵列。在IEF分析前，当RA血清用4- 6 Murea透析时，检测到额外的抗Fab抗体条带，表明血清中存在一些复合形式的抗Fab抗体。在这些解离条件下，也可以在正常受试者中检测到抗Fab抗体，但谱型模式比RA血清中的谱型模式更受限制。由于抗Fab抗体可以调节正常的免疫应答，RA中克隆限制性抗Fab抗体的数量增加可能表明这种免疫调节异常。
Clonally restricted anti‐IgG antibodies were detected, by isoelectric focusing (IEF) and chromatofocusing techniques, in the sera of patients with rheumatoid arthritis (RA). Anti‐Fab antibodies were predominantly acidic proteins with isoelectric points of 4.5–6.5 and displayed restricted spectrotype patterns. Proteins reactive with the Fc portion of IgG showed polyclonal spectrotype patterns with alkaline pI of 7.5–9.0. A limited array of anti‐Fab spectrotypes was consistently detected in RA sera when analyzed by IEF on 6Murea gels. Additional anti‐Fab antibody bands were detected when the RA sera were dialyzed against 4–6Murea prior to IEF analysis, indicating that some anti‐Fab antibodies exist in a complexed form in serum. Under these dissociating conditions, anti‐Fab antibodies could also be detected in normal subjects, but the spectrotype patterns were more restricted than those in RA sera. Because anti‐Fab antibodies may regulate normal immune responses, the increased quantity of clonally restricted anti‐Fab antibodies in RA may indicate an abnormality of this immunoregulation.