Bcl-2 expression correlates with apoptosis induction but not loss of clonogenic survival in small cell lung cancer cell lines treated with etoposide.
Bcl-2 expression correlates with apoptosis induction but not loss of clonogenic survival in small cell lung cancer cell lines treated with etoposide.
复制标题
在用依托泊苷处理的小细胞肺癌细胞系中，Bcl-2 表达与细胞凋亡诱导相关，但与克隆形成存活的丧失无关。
DOI：
10.1097/00001813-199810000-00002
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发表时间：
1998
影响因子：
2.3
通讯作者：
Meyn,RE
中科院分区：
文献类型：
--
作者：
Breton,C;Story,MD;Meyn,RE
在5个小细胞肺癌（SCLC）细胞系中研究了bcl-2癌基因表达对足叶乙甙诱导的凋亡和克隆形成存活的影响，其中3个细胞系表达bcf-2，2个细胞系不表达bcf-2。表达bcf-2的细胞系比不表达bcf-2的细胞系表现出更低的凋亡倾向。当bcf-2表达细胞在无胱氨酸/甲硫氨酸（CMF）培养基中孵育时，依托泊苷诱导的细胞凋亡恢复到与非bcf-2表达细胞系相当的水平。然而，药物治疗后的克隆生存终点并没有显示任何与bcf-2状态相关的一致模式。此外，在CMF培养基中用依托泊苷处理两种表达bcf-2的细胞系与在常规培养基中处理相比，没有改变它们的克隆形成存活曲线。这些结果与bcf-2表达调节足叶乙甙诱导的细胞凋亡但不调节克隆存活的观点一致。[(C)1998年利平科特威廉姆斯&威尔金斯。]
The influence of bcl-2 oncogene expression on etoposide-Induced apoptosis and clonogenic survival was investigated In five small cell lung cancer (SCLC) cell lines, three of which were bcf-2-expressing and two of which were non-bcf-2-expressing. The bcf-2-expressing lines displayed a lower apoptosis propensity than the non-bcf-2-expresslng lines. When bcf-2-expressing cells were incubated in cystine/methionine-free (CMF) medium, etoposide-induced apoptosis was restored to levels comparable to those seen in nonbcf-2-expressing lines. However, the endpoint of clonogenic survival after drug treatment did not display any consistent pattern that correlated with bcf-2 status. In addition, treatment of the two bcf-2-expressing cell lines with etoposide in CMF medium did not modify their clonogenic survival curves compared to treatment in regular medium. These results are consistent with the idea that bcf-2 expression modulates etoposide-induced apoptosis but not clonogenic survival.[(C) 1998 Lippincott Williams & Wllkins.]