Expression of perilipin 2 (PLIN2) in porcine oocytes during maturation.
Expression of perilipin 2 (PLIN2) in porcine oocytes during maturation.
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DOI：
10.1111/rda.12386
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Zhang Rn;Xiangwei Fu;Baoyu Jia;Cong Liu;Keren Cheng;Shi-En Zhu
中科院分区：
文献类型：
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作者：
Zhang Rn;Xiangwei Fu;Baoyu Jia;Cong Liu;Keren Cheng;Shi-En Zhu
据报道，周脂肪素可限制脂肪酶与液滴内中性脂质的相互作用，从而调节中性脂质的积累和利用。本研究旨在确定猪卵母细胞成熟过程中 PLIN1 和 PLIN2 的位置和表达。采用实时定量聚合酶链反应 (qRT-PCR)、免疫染色和蛋白质印迹方法来表征猪卵母细胞中 PLIN1 和 PLIN2 的表达和分布模式。结果显示，在猪卵母细胞中未检测到 PLIN1。 PLIN2 和 BODIPY 493/503 检测到的中性脂滴在 GV 和 MII 猪卵母细胞中表现出相同的分布模式和广泛的共定位。 GV 卵母细胞中的 PLIN2 蛋白表达高于 MII 卵母细胞 (p < 0.05)，尽管两组中 PLIN2 mRNA 表达相似。这些发现表明 PLIN2 是猪卵母细胞中主要的脂滴相关蛋白。
Perilipins have been reported to limit the interaction of lipases with neutral lipids within the droplets, thereby regulating neutral lipid accumulation and utilization. This study aimed to identify the location and expression of PLIN1 and PLIN2 in porcine oocytes during maturation. Quantitative real-time polymerase chain reaction (qRT-PCR), immunostaining and Western blot methods were used to characterize the expression and distribution patterns of PLIN1 and PLIN2 in porcine oocytes. The results showed that PLIN1 was not detectable in porcine oocytes. PLIN2 and BODIPY 493/503-detected neutral lipid droplets appeared identical distribution patterns and extensive colocalization in both GV and MII porcine oocytes. PLIN2 protein expression was higher in GV oocytes than that in MII oocytes (p < 0.05), although PLIN2 mRNA expression was similar in both groups. These findings suggested that PLIN2 was a major lipid droplet-associated protein in porcine oocytes.