Measurement and Comparison of Organic Compound Concentrations in Plasma, Whole Blood, and Dried Blood Spot Samples
Measurement and Comparison of Organic Compound Concentrations in Plasma, Whole Blood, and Dried Blood Spot Samples
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DOI：
10.3389/fgene.2016.00064
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发表时间：
2016-04-21
影响因子：
3.7
通讯作者：
Su, Feng-Chiao
中科院分区：
文献类型：
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作者：
Batterman, Stuart A.;Chernyak, Sergey;Su, Feng-Chiao
用于测量人体持久性有机化合物 (POP) 暴露量的首选采样介质是血液，相关样品类型包括全血、血浆和干血斑 (DBS)。由于有关这些样本类型的测量性能和可比性的信息有限，因此很难在研究之间进行比较。本研究评估了血浆、全血和 DBS 中 POP 测量的性能，并提出了在三种样品类型之间转换浓度所需的分配系数。从成年志愿者身上采集血样以及人口统计和吸烟信息，并通过 GC/MS 分析有机氯农药 (OCP)、氯代烃 (CHC)、多氯联苯 (PCB) 和溴化二苯醚 (PBDE)。回归模型用于评估样本类型与个人协变量的可能影响之间的关系。分配系数也是使用基于物理的模型计算的。在所有化合物中，血浆中的浓度始终最高；全血和 DBS 样本中的浓度相当。农药/CHC 的血浆到全血浓度的分配系数范围为 1.74 至 2.26，PCB 的平均为 1.69 +/- 0.06，PBDE 的平均为 1.65 +/- 0.03。回归模型非常适合大多数化学物质（R-2 > 0.80），全血和 DBS 样本通常表现出非常好的一致性。使用基于生物学的模型估计的分布系数接近于 1，并且不能解释观察到的分布。在研究人群中，几种农药/CHC 和 PBDE 的中值浓度超过了 2007-2008 年国家健康和营养调查报告中报告的水平，而其他 OCP 和 PBDE 的水平相当或更低。种族和吸烟状况似乎对几种持久性有机污染物的血浆/血液浓度比略有影响。实验确定的分布系数可用于比较使用不同类型血液基质的研究中的 POP 暴露。
The preferred sampling medium for measuring human exposures of persistent organic compounds (POPs) is blood, and relevant sample types include whole blood, plasma, and dried blood spots (DBS). Because information regarding the performance and comparability of measurements across these sample types is limited, it is difficult to compare across studies. This study evaluates the performance of POP measurements in plasma, whole blood and DBS, and presents the distribution coefficients needed to convert concentrations among the three sample types. Blood samples were collected from adult volunteers, along with demographic and smoking information, and analyzed by GC/MS for organochlorine pesticides (OCPs), chlorinated hydrocarbons (CHCs), polychlorinated biphenyls (PCBs), and brominated diphenyl ethers (PBDEs). Regression models were used to evaluate the relationships between the sample types and possible effects of personal covariates. Distribution coefficients also were calculated using physically-based models. Across all compounds, concentrations in plasma were consistently the highest; concentrations in whole blood and DBS samples were comparable. Distribution coefficients for plasma to whole blood concentrations ranged from 1.74 to 2.26 for pesticides/CHCs, averaged 1.69 +/- 0.06 for the PCBs, and averaged 1.65 +/- 0.03 for the PBDEs. Regression models closely fit most chemicals (R-2 > 0.80), and whole blood and DBS samples generally showed very good agreement. Distribution coefficients estimated using biologically-based models were near one and did not explain the observed distribution. Among the study population, median concentrations of several pesticides/CHCs and PBDEs exceeded levels reported in the 2007-2008 National Health and Nutrition Examination Survey, while levels of other OCPs and PBDEs were comparable or lower. Race and smoking status appeared to slightly affect plasma/blood concentration ratios for several POPs. The experimentally-determined distribution coefficients can be used to compare POP exposures across studies using different types of blood-based matrices.