STUDIES OF THE BIOSYNTHESIS OF 3,6-DIDEOXYHEXOSES - MOLECULAR-CLONING AND CHARACTERIZATION OF THE ASC (ASCARYLOSE) REGION FROM YERSINIA-PSEUDOTUBERCULOSIS SEROGROUP VA
STUDIES OF THE BIOSYNTHESIS OF 3,6-DIDEOXYHEXOSES - MOLECULAR-CLONING AND CHARACTERIZATION OF THE ASC (ASCARYLOSE) REGION FROM YERSINIA-PSEUDOTUBERCULOSIS SEROGROUP VA
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DOI：
10.1128/jb.176.17.5483-5493.1994
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发表时间：
1994-09-01
影响因子：
3.2
通讯作者：
LIU, HW
中科院分区：
文献类型：
--
作者：
THORSON, JS;LO, SF;LIU, HW
在革兰氏阴性细菌的脂多糖中发现了3，6-二脱氧己糖，它们已被证明是主要的抗原决定因素。在已知的五种自然产生的3，6-二脱氧己糖中，有四种存在于不同的肠沙门氏菌株中(甲壳糖、酪威糖、副糖和肠杆菌糖)，包括子囊糖在内的所有五种都存在于假结核耶尔森菌的血清型中。尽管已有一份克隆假结核耶尔森菌Rfb区的报道，该区域含有假结核耶尔森菌IIA亚群的双糖生物合成基因，但其3，6-二脱氧己糖多态的详细遗传学原理尚未得到解决。扩展耶尔森氏菌3，6-二脱氧己糖形成相关基因的现有信息。并便于与已建立的沙门氏菌RFB(0抗原)簇进行比较，我们报告了三个重叠克隆的产生，其中包含CDP-子囊糖生物合成所需的整个基因簇。在详细序列分析的基础上，探讨了沙门氏菌和耶尔森氏菌之间3，6-二脱氧己糖多态性的意义。并对其进行了讨论。此外，该区域的功能克隆还允许表达E(P)(α-D-葡萄糖胞苷转移酶)、E(Od)(CDP-D-葡萄糖4，6-脱水酶)、E(1)(CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase)E(3)(CDP-6-脱氧-三角洲(3，4)-葡萄糖素还原酶)、E(EP)(CDP-3，6-dideoxy-D-glycero-D-glycero-4-hexulose-5-epimerase)，和E(红色)(CDP-3，6-dideoxy-L-glycero-D-glycero-4-hexulose-4-reductase)，为这一有趣的生物合成途径的未来机制研究提供了便利。
The 3,6-dideoxyhexoses are found in the lipopolysaccharides of gram-negative bacteria, where they have been shown to be the dominant antigenic determinants. Of the five 3,6-dideoxyhexoses known to occur naturally four have been found in various Strains of Salmonella enterica (abequose, tyvelose, paratose, and colitose) and all five, including ascarylose, are present among the serotypes of Yersinia pseudotuberculosis. Although there exists one report of the cloning of the rfb region;harboring the abequose biosynthetic genes from Y. pseudotuberculosis serogroup IIA, the detailed genetic principles underlying a 3,6-dideoxyhexose polymorphism in Y. pseudotuberculosis have not been addressed. To extend the available information on the genes responsible for 3,6-dideoxyhexose formation in Yersinia spp. and facilitate a comparison with the established rfb (0 antigen) cluster of Salmonella spp;, we report the production of three overlapping clones containing the entire gene cluster required for CDP-ascarylose biosynthesis. On the basis of a detailed sequence analysis, the implications regarding 3,6-dideoxyhexose polymorphism among Salmonella and Yersinia spp. are discussed. In addition, the functional cloning of this region has allowed the expression of E(p) (alpha-D-glucose cytidylyltransferase), E(od) (CDP-D-glucose 4,6-dehydratase), E(1) (CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase) E(3) (CDP-6-deoxy-Delta(3,4)-glucoseen reductase), E(ep) (CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose-5-epimerase), and E(red) (CDP-3,6-dideoxy-L-glycero-D-glycero-4-hexulose-4-reductase), facilitating future mechanistic studies of this intriguing biosynthetic pathway.