Differentiation stage alters matrix control of stem cells
Differentiation stage alters matrix control of stem cells
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DOI：
10.1002/jbm.a.31521
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发表时间：
2008-04-01
影响因子：
4.9
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Hsiong, Susan X.;Carampin, Paolo;Mooney, David J.
细胞粘附的材料（例如，粘附配体的呈现，底物弹性模量）明显影响分化细胞的表型。然而，目前尚不清楚干细胞是否对这些信号有类似的反应。本研究考察了不同硬度水凝胶中模型细胞粘附配体（含精氨酸-甘氨酸-天氨酸[RGD]肽）的总体密度和纳米级组织如何调节克隆来源的干细胞系（D1细胞）和前成骨细胞（MC3T3-E1）的增殖。MC3T3-E1成骨前细胞的生长速度对纳米级RGD配体组织和底物硬度有响应，而D1干细胞在未提交状态下对这些线索不太敏感。然而，一旦D1细胞向成骨细胞谱系分化，它们对这些信号的反应变得更加敏感。这些结果表明，细胞对材料线索的反应取决于细胞承诺或分化的阶段，这些发现可能会影响组织再生生物材料的设计。(c) 2007 Wiley期刊公司
Cues from the material to which a cell is adherent (e.g., adhesion ligand presentation, substrate elastic modulus) clearly influence the phenotype of differentiated cells. However, it is currently unclear if stem cells respond similarly to these cues. This study examined how the overall density and nanoscale organization of a model cell adhesion ligand (arginine-glycine-aspartic acid [RGD] containing peptide) presented from hydrogels of varying stiffness regulated the proliferation of a clonally derived stem cell line (D1 cells) and preostcoblasts (MC3T3-E1). While the growth rate of MC3T3-E1 preosteoblasts was responsive to nanoscale RGD ligand organization and substrate stiffness, the D1 stem cells were less sensitive to these cues in their uncommitted state. However, once the D1 cells were differentiated towards the osteoblast lineage, they became more responsive to these signals. These results demonstrate that the cell response to material cues is dependent on the stage of cell commitment or differentiation, and these findings will likely impact the design of biomaterials for tissue regeneration. (c) 2007 Wiley Periodicals, Inc.