Characterization of DegU-dependent expression of bpr in Bacillus subtilis
Characterization of DegU-dependent expression of bpr in Bacillus subtilis
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DOI：
10.1111/j.1574-6968.2007.01019.x
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发表时间：
2008-03-01
影响因子：
2.1
通讯作者：
Ogura, Mitsuo
中科院分区：
文献类型：
--
作者：
Tsukahara, Kensuke;Ogura, Mitsuo
在革兰氏阳性土壤细菌枯草芽孢杆菌中，反应调节因子DEGU和它的同源组氨酸蛋白激酶DEGS构成了一个双组分系统。已知的磷酸化形式的DECU可以激活枯草杆菌中120多个基因的转录，其中包括编码杆菌肽酶F的BPR基因。为了表征BPR对DECU的依赖调节，我们使用凝胶延迟和足迹分析方法分析了BPR调节区与His标记的DECU的相互作用。这表明，Degu结合了一个基序的三个直接重复，该基序在comK启动子中以反向重复的形式排列，并与之结合。用BPR-LacZ融合进行的突变分析表明，BPR中的三个直接重复序列是DECU依赖的转录激活所必需的。
The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. The phosphorylated form of DegU is known to activate transcription of more than 120 genes in B. subtilis, including the bpr gene encoding bacillopeptidase F. To characterize DegU-dependent regulation of bpr, the interaction of the bpr regulatory region with His-tagged DegU was analyzed using gel retardation and footprint analyses. This revealed that DegU bound three direct repeats of a motif that is known to be arranged as an inverted repeat in the comK promoter, to which DegU binds. Mutational analysis using a bpr-lacZ fusion revealed that the three direct repeats in bpr are needed for DegU-dependent transcription activation.