Upregulation of heme oxygenase and collagen type III in the rat bladder after partial bladder outlet obstruction
Upregulation of heme oxygenase and collagen type III in the rat bladder after partial bladder outlet obstruction
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DOI：
10.1159/000099351
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发表时间：
2007-01-01
影响因子：
1.6
通讯作者：
Miki, Tsuneharu
中科院分区：
文献类型：
--
作者：
Inaba, Mitsuhiko;Ukimura, Osamu;Miki, Tsuneharu
本研究的目的是评估可能的变化的基因表达和本地化的酶，血红素加氧酶和一氧化氮合酶（NOS），参考增加的III型胶原蛋白的膀胱部分梗阻。在最初的阻塞，整个大鼠膀胱取出实时定量逆转录聚合酶链反应（RT-PCR）和免疫组化。实时定量RT-PCR显示术后第14天HO（p < 0.01）和III型胶原（p < 0.001）基因表达显著增强。NOS基因表达仅在术后第4天增强（p < 0.01）。免疫组化结果显示，HO-1的免疫反应在神经细胞和神经纤维中有很多共同点，而HO-2和iNOS的免疫反应相对较弱。本研究提示梗阻后HO基因表达的变化较NOS更为明显，HO-1的表达与III型胶原的表达同步上调。HO系统可能参与了梗阻后III型胶原增加相关的膀胱功能障碍的发病机制。版权所有（c）2007 S. Karger AG，巴塞尔
The objective of the study was to evaluate possible changes of the gene expression and localization of the enzymes, heme oxygenase and nitric oxide synthase (NOS), with reference to increase of collagen type III in response to the partial obstruction of the bladder. Following initial obstruction, whole rat bladders were removed for real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Real-time RT-PCR demonstrated significantly enhanced expression of HO (p < 0.01) and collagen type III (p < 0.001) gene on postoperative day 14. Enhanced expression of NOS gene was seen only on postoperative day 4 (p < 0.01). Immunohistochemistry revealed that immunoreactivity to HO-1 had much in common in neural cells and fibers, although immunoreactivity to HO-2 and iNOS was relatively weak. This study suggested gene expression of HO, especially HO-1, was more dramatically changed than NOS, and was upregulated simultaneously with increase of collagen type III after obstruction. HO systems could be involved in the pathogenesis of bladder dysfunction related to increase of collagen type III after obstruction. Copyright (c) 2007 S. Karger AG, Basel