High fat diet induces ceramide and sphingomyelin formation in rat's liver nuclei
High fat diet induces ceramide and sphingomyelin formation in rat's liver nuclei
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DOI：
10.1007/s11010-010-0409-6
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发表时间：
2010-07-01
影响因子：
4.3
通讯作者：
Gorski, Jan
中科院分区：
文献类型：
--
作者：
Chocian, Grzegorz;Chabowski, Adrian;Gorski, Jan
肥胖会增加患肝脂肪变性的风险。近期研究表明，高脂肪饮食（HFD）可能会影响骨骼肌、心脏和其他组织中鞘脂的形成。在这项研究中，我们试图探究高脂肪饮食是否会引起肝脏和肝细胞核层面的鞘磷脂和神经酰胺含量以及脂肪酸（FAs）组成的变化。此外，我们还研究了神经酰胺的形成是否与中性鞘磷脂酶（N - SMase）或酸性鞘磷脂酶（A - SMase）的活性有关。给予三周的高脂肪饮食导致肝脏和肝细胞核中神经酰胺和鞘磷脂显著积累，同时中性鞘磷脂酶活性增加，而酸性鞘磷脂酶活性未增加。此外，还观察到这些鞘脂的脂肪酸饱和度状态向更高水平转变。这些发现支持这样的结论：高脂肪饮食不仅对肝脏中的鞘脂代谢有重大影响，对肝细胞核中的鞘脂代谢也有重大影响。
Obesity increases the risk for hepatic steatosis. Recent studies have demonstrated that high fat diet (HFD) may affect sphingolipid formation in skeletal muscles, heart, and other tissues. In this work we sought to investigate whether HFD feeding provokes changes in content and fatty acids (FAs) composition of sphingomyelin and ceramide at the level of liver and hepatic nuclei. Furthermore, we investigated whether the ceramide formation is related to the activity of either neutral sphingomyelinase (N-SMase) or acidic sphingomyelinase (A-SMase). Three weeks of HFD provision induced pronounced ceramide and sphingomyelin accumulation in both liver and hepatic nuclei, accompanied by increased activity of N-SMase but not A-SMase. Furthermore, a shift toward greater FAs saturation status in these sphingolipids was also observed. These findings support the conclusion that HFD has a major impact on sphingolipid metabolism not only in the liver, but also in hepatic nuclei.