Expression of AMT1;1 and AMT2;1 is stimulated by mineral nitrogen and reproductive growth stage in barley under field conditions
Expression of AMT1;1 and AMT2;1 is stimulated by mineral nitrogen and reproductive growth stage in barley under field conditions
复制标题
DOI：
10.1080/01904167.2022.2067764
复制
发表时间：
2022-04-19
影响因子：
2.1
通讯作者：
Loit, Evelin
中科院分区：
文献类型：
--
作者：
Esmaeilzadeh-Salestani, Keyvan;Samandari-Bahraseman, Mohammad Rasoul;Loit, Evelin
大麦是重要的粮食和饲料作物。氮（N）是植物生产力的关键元素。氮素管理需要大量的资金和精力，过量施用会造成环境危害。因此，未来的品种和管理系统需要更有效地吸收和利用氮素。铵转运蛋白（AMTs）是氮素吸收和转运的重要基因。由于氮对生物量生产有很大的影响，因此假设在田间条件下，施氮量和大麦生长阶段对AMT表达有显着影响。于2019年进行田间试验，以确定HvAMT 1;1和HvAMT 2;1基因在不同氮肥用量和类型下不同生育期的表达模式。HvAMT 1;1和HvAMT 2;1在抽穗期表达下调，开花期表达上调，其中无机肥的作用较强。HvAMT 1;1的表达量是HvAMT 2;1的2.8倍。AMT基因的表达量与开花期叶片含氮量和千粒重均呈正相关。总之，氮的吸收和转运部分由HvAMT 1;1和HvAMT 2;1控制，这是一个有价值的候选育种计划。
Barley (Hordeum vulgare) is an important cereal for providing food and feed. Nitrogen (N) is a crucial element for plant productivity. N fertilizing management requires large amount of money and energy, and excess application makes environmental hazards. Therefore, the future varieties and management systems need to be more efficient in nitrogen uptake and utilization. Ammonium transporters (AMTs) are important genes in N uptake and transport. Since N has strong impact on biomass production, it was hypothesized that N rate and barley growth stage have significant influence on AMT expression under field conditions. The field experiment was carried out in 2019 to determine expression patterns of HvAMT1;1 and HvAMT2;1 genes at different growth stages under different N fertilizer rates and types. A down-regulation of HvAMT1;1 and HvAMT2;1 until heading and up-regulation at anthesis stage was observed compared to the unfertilized treatment, mineral fertilizers having stronger effect. HvAMT1;1 was expressed 2.8 times more than HvAMT2;1 at the same time. There was a positive correlation between both AMT genes expression and N content in leaves at anthesis as well as 1000-kernel weight. In conclusion, nitrogen uptake and translocation is partially governed by HvAMT1;1 and HvAMT2;1, and it is a valuable candidate for breeding programs.