Enhanced Probiotic Potential of Lactobacillus reuteri When Delivered as a Biofilm on Dextranomer Microspheres That Contain Beneficial Cargo.
Enhanced Probiotic Potential of Lactobacillus reuteri When Delivered as a Biofilm on Dextranomer Microspheres That Contain Beneficial Cargo.
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DOI：
10.3389/fmicb.2017.00489
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发表时间：
2017
影响因子：
5.2
通讯作者：
Goodman SD
中科院分区：
文献类型：
--
作者：
Navarro JB;Mashburn-Warren L;Bakaletz LO;Bailey MT;Goodman SD
与所有口服益生菌一样，革兰氏阳性菌罗伊氏乳杆菌在通过宿主胃肠道时遇到许多挑战，包括低pH值，宿主免疫系统的效应，以及与共生菌和致病菌的竞争，所有这些都会大大减少用于治疗目的的活菌的可用性。最近，我们发现罗伊氏乳杆菌以生物膜的形式粘附在半透性生物相容性右旋异构体微球上，在一个明确定义的动物模型中，在给予单次预防性剂量后，可将坏死性小肠结肠炎的发生率降低50%。在此，我们使用相同的半透性微球，我们发现在微球管腔内提供对罗伊氏乳杆菌有益的化合物作为扩散货物，产生了进一步的有利效应，包括葡萄糖基转移酶依赖的细菌对微球表面的粘附，结合细菌对酸性条件的抗性，增强罗伊氏乳杆菌对体外人肠上皮细胞的粘附。并促进了抗菌化合物罗伊特素和抗炎分子组胺的产生。这些数据支持这种新型益生菌制剂的持续发展，作为一种适应性强且有效的手段，用于靶向输送对益生菌有益的货物。
As with all orally consumed probiotics, the Gram-positive bacterium Lactobacillus reuteri encounters numerous challenges as it transits through the gastrointestinal tract of the host, including low pH, effectors of the host immune system, as well as competition with commensal and pathogenic bacteria, all of which can greatly reduce the availability of live bacteria for therapeutic purposes. Recently we showed that L. reuteri, when adhered in the form of a biofilm to a semi-permeable biocompatible dextranomer microsphere, reduces the incidence of necrotizing enterocolitis by 50% in a well-defined animal model following delivery of a single prophylactic dose. Herein, using the same semi-permeable microspheres, we showed that providing compounds beneficial to L. reuteri as diffusible cargo within the microsphere lumen resulted in further advantageous effects including glucosyltransferase-dependent bacterial adherence to the microsphere surface, resistance of bound bacteria against acidic conditions, enhanced adherence of L. reuteri to human intestinal epithelial cells in vitro, and facilitated production of the antimicrobial compound reuterin and the anti-inflammatory molecule histamine. These data support continued development of this novel probiotic formulation as an adaptable and effective means for targeted delivery of cargo beneficial to the probiotic bacterium.