Spectrally filtered photodiode pairs for on-chip ratiometric aptasensing of cytokine dynamics
Spectrally filtered photodiode pairs for on-chip ratiometric aptasensing of cytokine dynamics
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DOI：
10.1016/j.snb.2021.130330
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发表时间：
2021-10
影响因子：
8.4
通讯作者：
Z. Xiong;Kewei Ren;M. Donnelly;M. You;Guangyu Xu
中科院分区：
文献类型：
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作者：
Z. Xiong;Kewei Ren;M. Donnelly;M. You;Guangyu Xu
小型化、高性价比的细胞因子传感装置对于急性免疫反应患者的健康监测和重症监护至关重要。在这里，我们报告了一种芯片上的比例感应装置，用于监测基本免疫反应生物标志物干扰素- γ的动态。我们的装置是由一对光谱过滤的硅光电二极管组成的，它们的表面功能化与适体探针。这些光电二极管对能够快速检测由细胞内适配体-细胞因子结合事件转导的双色荧光变化。6分钟在皮摩尔水平，这是足以捕捉细胞因子瞬态在护理点设置。此外，我们的设备可以通过简单的洗涤步骤重置到基线，在连续运行中产生一致的读数。结合其芯片级结构和实时操作，我们的细胞因子传感器可能在护理点和治疗筛选应用中使用。
Miniaturized, cost-effective cytokine sensing devices are essential for health monitoring and critical care of patients with acute immune responses. Here we report an on-chip ratiometric aptasensing device to monitor the dynamics of an essential immune response biomarker, interferon-gamma. Our device is formed by pairs of spectrally filtered silicon photodiodes that are surface functionalized with aptamer probes. These photodiode pairs are able to rapidly detect two-color fluorescence changes transduced from aptamer-cytokine binding events withinc.a.6 min at picomolar levels, which are sufficient to capture the cytokine transients in point-of-care settings. Furthermore, our device can be reset to baseline by simple washing steps, yielding consistent readout in consecutive runs. Combined with their chip-scale construct and real-time operation, our cytokine sensors may have possible use in point-of-care and therapeutic screening applications.