Characterization of Secretory and Morphologic Properties of Primary Cultured Endocrine Cells from Porcine Pancreata
Characterization of Secretory and Morphologic Properties of Primary Cultured Endocrine Cells from Porcine Pancreata
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原代培养的猪胰腺内分泌细胞的分泌和形态特性的表征
DOI：
10.1097/00006676-200103000-00005
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
I. Niki
中科院分区：
文献类型：
--
作者：
T. Niwa;T. Fukasawa;Wei Yu;Y. Nimura;T. Senda;H. Ohgawara;I. Niki
我们的特点是形态和分泌特性的猪胰腺内分泌细胞在原代培养获得的自溶制备无任何外源性蛋白水解酶。内分泌细胞呈神经元样，突起末端有胰岛素颗粒堆积。因此，衍生的内分泌细胞在含有烟酰胺的培养基中存活，并在制备后至少6周内对葡萄糖保持敏感。细胞对生理浓度的葡萄糖反应良好，高K+去极化和抗糖尿病磺脲类、甲苯磺丁脲和格列本脲也引起释放。在高糖条件下，毛喉素、胰高血糖素、胰高血糖素样肽-1和精氨酸显著增强胰岛素释放，生长抑素、钙通道阻滞剂尼群地平和ATP敏感性K+通道开放剂二氮嗪抑制胰岛素释放。肾上腺素对葡萄糖的释放有双重作用，在低纳摩尔浓度范围内增强，在1 mol/L时抑制。然而，这些细胞对亮氨酸没有反应。这些细胞对营养素、激素和药理学试剂的这种分泌敏感性以及长存活率（长达5-6周）因此向我们暗示，衍生的内分泌细胞可用于胰腺β细胞的异种移植以治疗胰岛素依赖型糖尿病。
We characterized morphologic and secretory properties of porcine pancreatic endocrine cells in primary culture obtained by autolytic preparation without any exogenous proteolytic enzymes. The endocrine cells exhibited a neuron-like shape, and insulin granules were accumulated at the terminal of the processes. Thus derived endocrine cells survived in culture medium containing nicotinamide and remained sensitive to glucose for at least 6 weeks after preparations. The cells responded well to physiologic concentrations of glucose, and high K+ depolarization and the antidiabetic sulfonylureas, tolbutamide, and glibenclamide also elicited the release. With high glucose, insulin release was markedly potentiated by forskolin, glucagon, glucagon-like peptide-1, and arginine and inhibited by somatostatin, the Ca2+ channel blocker nitrendipine, and the ATP-sensitive K+ channel opener diazoxide. Epinephrine had dual effects on the release by glucose; enhanced within a low nanomolar range and inhibited at 1 &mgr;mol/L. However, the cells were unresponsive to leucine. Such secretory sensitivities to nutrients, hormones, and pharmacologic agents, and long survival rate (as long as 5–6 weeks) of these cells suggest to us therefore that derived endocrine cells may be useful for xenotransplantation of pancreatic beta cells for treatment of insulin-dependent diabetes mellitus.
影响因子：
20.3
作者：
T. Kieffer;J. Habener
通讯作者：
T. Kieffer;J. Habener