Conversion of chlorinated volatile organic compounds to carbon dioxide and methyl chloride for isotopic analysis of carbon and chlorine
Conversion of chlorinated volatile organic compounds to carbon dioxide and methyl chloride for isotopic analysis of carbon and chlorine
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DOI：
10.1021/ac961096b
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发表时间：
1997-07-15
影响因子：
7.4
通讯作者：
Heraty, LJ
中科院分区：
文献类型：
--
作者：
Holt, BD;Sturchio, NC;Heraty, LJ
本文介绍了一种非水高温法，用于将微摩尔量的氯化挥发性有机化合物转化为CO_2和CH_3Cl，以测定C和Cl同位素比值。该方法为利用碳和氯同位素比值研究环境中氯代挥发性有机化合物的生物降解提供了一种改进的分析方法。通过本发明的方法，将试剂CH 3Cl转化为CO2 + CuCl，然后将CuCl转化回CH 3Cl，得到典型的CO2产率为99 +/- 1%，CH 3Cl产率为91 +/- 1%，两种产物的纯度均>99%。在产物和初始CH 3Cl的Cl-37/Cl-35比率之间观察到-0.23 +/- 0.05 ppm的偏移。对于各种氯化挥发性有机化合物，同位素比率测量的精度优于+/- 0.1千分之一。
A nonaqueous, high-temperature method is described for converting micromole quantities of chlorinated volatile organic compounds to CO2 and CH3Cl for C and Cl isotope ratio determinations. This method provides an improved analytical approach for using C and CI isotope ratios in studies of the biodegradation of chlorinated volatile organic compounds in the environment. Conversion of reagent CH3Cl to CO2 + CuCl and then conversion of the CuCl back to CH3Cl by the present method gives typical yields of 99 +/- 1% for CO2 and 91 +/- 1% for CH3Cl, both products of >99% purity. An offset of -0.23 +/- 0.05 parts per thousand is observed between the Cl-37/Cl-35 ratios of product and initial CH3Cl. Precision of the isotopic ratio measurements is better than +/- 0.1 parts per thousand for a variety of chlorinated volatile organic compounds.