Morphological and molecular characterization of the metacercaria of Paragonimus caliensis, as a separate species from P. mexicanus in Costa Rica
Morphological and molecular characterization of the metacercaria of Paragonimus caliensis, as a separate species from P. mexicanus in Costa Rica
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DOI：
10.1016/j.parint.2016.12.006
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发表时间：
2017-04-01
影响因子：
1.9
通讯作者：
Dolz, Gaby
中科院分区：
文献类型：
--
作者：
Hernandez-Chea, Roderico;Eugenia Jimenez-Rocha, Ana;Dolz, Gaby
墨西哥并殖吸虫是拉丁美洲一种食源性人畜共患病的病原体。本种，以及并殖吸虫，已从哥斯达黎加被报告，但不知道这两个是否同义。在光镜下，从哥斯达黎加几个地区的淡水蟹中检出两种并殖吸虫囊虫。在形态上，这些与墨西哥拟青霉和加州拟青霉的描述相一致。前一种的囊虫无膜或囊，体色为黄色。卡氏肺孢子虫的卵囊呈粉红色，内含透明的薄囊。用扫描电子显微镜(SEM)对囊尾蚴进行了形态分型。根据腹吸器中乳突的数量和分布，发现墨西哥对虾有3种形态类型，而卡氏对虾有2种形态类型。DNA序列分析(核糖体28S和ITS2基因，以及部分线粒体cox1基因)证实了墨西哥疫霉的存在，并提供了加州疫霉的第一个分子数据。这两个物种在系统发育上彼此不同，与亚洲物种相距甚远。卡氏并殖吸虫被确认为独立于墨西哥并殖吸虫的独立种，引发了关于哥斯达黎加并殖吸虫属的生态学、生物多样性和流行病学的几个问题。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
The trematode Paragonimus mexicanus is the etiological agent of paragonimiasis, a food-borne zoonotic disease in Latin America. This species, as well as Paragonimus caliensis, have been reported from Costa Rica, but it is not known if the two are synonymous. Two types of Paragonimus metacercariae from freshwater pseudothelphusid crabs from several localities in Costa Rica were recognized by light microscopy. Morphologically, these corresponded to descriptions of P. mexicanus and P. caliensis. Metacercariae of the former species lacked a membrane or cyst and their bodies were yellow in color. Those of P. caliensis were contained in a transparent thin cyst and were pink in color. Morphotypes of metacercariae were determined using scanning electron microscopy (SEM). Based on the number and distribution of papillae in the ventral sucker, three morphotypes were found for P. mexicanus and two for P. caliensis. Analysis of DNA sequences (nuclear ribosomal 28S and ITS2 genes, and partial mitochondrial coxl gene) confirmed the presence of P. mexicanus and provided the first molecular data for P. caliensis. The two species are phylogenetically distinct from each other and distant from the Asian species. The confirmation of P. caliensis as a separate species from P. mexicanus raises several questions about the ecology, biological diversity, and epidemiology of the genus Paragonimus in Costa Rica. (C) 2016 Elsevier Ireland Ltd. All rights reserved.