Cerebrospinal fluid-contacting neurons affect the expression of endogenous neural progenitor cells and the recovery of neural function after spinal cord injury
Cerebrospinal fluid-contacting neurons affect the expression of endogenous neural progenitor cells and the recovery of neural function after spinal cord injury
复制标题
脑脊液接触神经元影响脊髓损伤后内源性神经祖细胞的表达和神经功能的恢复
DOI：
10.1080/00207454.2020.1750396
复制
发表时间：
2020-04-15
影响因子：
2.2
通讯作者：
Li, Qing
中科院分区：
文献类型：
--
作者：
He, Yu-qi;Shi, Xue-xing;Li, Qing
目的：探讨脊髓损伤后接触脑脊液神经元（CSF-cNs）与内源性神经前体细胞（ENPCs）的关系及CSF-cNs是否参与神经修复。研究方法：将霍乱毒素B-辣根过氧化物酶复合物（CB-HRP）和霍乱毒素B-皂草素偶联物（CB-SAP）注入脊髓损伤大鼠侧脑室，标记并破坏CSF-cNs。实验组大鼠采用脊髓损伤模型。观察各组大鼠CSF-cNs和ENPC的含量及共表达，观察各组SCI后运动功能的恢复情况。结果如下：CSF-cNs破坏后，ENPC的数量在术后的长期内显著减少，并且运动功能的恢复也比CSF-cNs完整的组恶化。同时，脊髓中的一些细胞同时表达CSF-cNs和ENPCs的生物学标记。结论：本研究表明，CSF-cNs破坏后，SCI大鼠ENPC数量和运动功能恢复下降，表明CSF-cNs影响ENPC数量，并可能参与SCI后神经功能的恢复。
Objective: To explore the relationship between cerebrospinal fluid-contacting neurons (CSF-cNs) and endogenous neural progenitor cells (ENPCs) and whether CSF-cNs are involved in nerve repair after spinal cord injury (SCI). Methods: Cholera toxin B-horseradish peroxidase complex (CB-HRP) and cholera toxin B conjugated with saporin (CB-SAP) were injected into the lateral ventricles of spinal cord injured rats to mark and destroy the CSF-cNs. Then the rats in the experimental group were injured by SCI. Observe the content and co-expression of CSF-cNs and ENPCs in rats of each group, and observe the recovery of motor function after SCI in each group. Results: After the destruction of CSF-cNs, the number of ENPCs decreased significantly in the long term after the surgery, and the recovery of motor function also deteriorated as compared to the group with intact CSF-cNs. Meanwhile some cells in the spinal cord express both the biological marker of CSF-cNs and ENPCs. Conclusion: This study shows that the population of ENPCs and motor function recovery in SCI rats declined after the destruction of CSF-cNs, suggesting that CSF-cNs affect the ENPCs population and may be involved in the recovery of neural function after SCI.