Biocontrol potential of Myxococcus sp strain BS against bacterial soft rot of calla lily caused by Pectobacterium carotovorum
Biocontrol potential of Myxococcus sp strain BS against bacterial soft rot of calla lily caused by Pectobacterium carotovorum
复制标题
粘球菌属的生物防治潜力。
DOI：
10.1016/j.biocontrol.2018.07.004
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发表时间：
2018-11-01
影响因子：
4.2
通讯作者：
Fan, Jiaqin
中科院分区：
文献类型：
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作者：
Li, Zhoukun;Wang, Ting;Fan, Jiaqin
由胡萝卜软腐果胶杆菌胡萝卜亚种引起的细菌性软腐病是马蹄莲（马蹄莲属）的一种毁灭性植物病害。考虑到马蹄莲在切花产业中的经济价值，迫切需要有效的防治这种病害的方法。对12个菌株的筛选得到菌株BS是对抗胡萝卜软腐果胶杆菌的最佳拮抗物。这个具有优异抗菌活性的菌株被鉴定为属于粘球菌属。进一步的研究表明，涉及细胞外囊泡的捕食者 - 猎物相互作用参与了抗菌行为，并且有效的捕食以及随后子实体的形成是BS在土壤中的适应和定殖能力的原因。盆栽试验表明，菌株BS将马蹄莲软腐病的发病率从75.0%显著降低到19.8%，病情指数从62.5%降低到6.3%。当菌株BS施用于土壤时，经过预处理的马蹄莲植株比未处理的对照表现出显著更高的生长水平和更多的子代块茎数量。由于其多种有益特性，菌株BS有潜力被开发成为一种用于综合病害和健康管理策略的植物生长促进和生物防治剂。
Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum is a devastating plant disease in calla lily (Zantedeschia spp.). Considering the economic value of calla lily in the cut flower industry, effective means for controlling this disease are urgently needed. A screening of 12 isolates yielded strain BS as the best antagonist against P. carotovorum. The isolate with excellent antibacterial activity was identified as belonging to Myxococcus sp. Further research indicated that predator-prey interactions involving extracellular vesicles are involved in the antibacterial behavior, and that efficient predation followed by fruiting body formation was responsible for the adaption and colonization ability of BS in soil. Pot experiments showed that strain BS significantly decreased the incidence of soft rot of calla lily to 19.8% from 75.0%, and the disease index was lowered to 6.3% from 62.5%. When the strain BS was applied to the soil, the pre-treated calla lily plants displayed significantly higher levels of growth and greater number of progeny tubers than untreated controls. Due to its multiple beneficial traits, strain BS has the potential to be developed into a plant growth promoting and biocontrol agent for integrated disease and health management strategies.