Linolenic acid improves oocyte developmental competence and decreases apoptosis of in vitro-produced blastocysts in goat
Linolenic acid improves oocyte developmental competence and decreases apoptosis of in vitro-produced blastocysts in goat
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亚麻酸可改善山羊卵母细胞的发育能力并减少体外产生的囊胚的凋亡
DOI：
10.1017/s0967199415000507
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发表时间：
2015
期刊：
影响因子：
1.7
通讯作者：
E. Gastal
中科院分区：
文献类型：
--
作者：
A. Veshkini;A. Khadem;A. Mohammadi;A. A. Alamouti;M. Soleimani;E. Gastal
本研究探讨了α-亚麻酸（ALA）对山羊卵母细胞发育能力的影响。最初，小窦卵泡和大窦卵泡中的ALA水平测定为0.018-0.028 mg/ml（分别为64.6-100.6 μM）。在存在不同浓度（10、50、100或200 μM）ALA的情况下进行体外成熟。卵丘扩张，减数分裂成熟，细胞内谷胱甘肽（GSH）水平，胚胎卵裂，孤雌激活（PA）和体外受精（IVF）后的囊胚形成，囊胚中的总细胞数和凋亡细胞数，以及囊胚细胞中Bax，Bcl-2和p53基因的表达进行了测定。与对照组相比，ALA处理组的卵丘扩张没有观察到改善。在50 μM浓度下，ALA可提高减数分裂成熟率，但对GSH水平无影响。当用50 μM ALA处理的卵母细胞随后用于PA或IVF时，观察到较高的囊胚形成率，并且这些胚胎具有较高的总细胞数和较低的凋亡细胞数。囊胚基因表达分析显示，50 μM ALA组Bax基因转录本丰度较低，Bcl-2基因转录本丰度较高。ALA处理的囊胚中p53基因的表达也较少。结果表明，在山羊卵母细胞体外成熟（IVM）过程中，50 μM浓度的ALA处理对卵母细胞的成熟有促进作用，并进而刺激胚胎发育和调节凋亡基因的表达。
Summary The effects of α-linolenic acid (ALA) on developmental competence of oocytes in goats were evaluated in this study. Initially, the level of ALA in small and large antral follicles was determined to be in a range of 0.018–0.028 mg/ml (64.6–100.6 μM, respectively). In vitro maturation was performed in the presence of various concentrations (10, 50, 100, or 200 μM) of ALA. Cumulus expansion, meiotic maturation, levels of intracellular glutathione (GSH), embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), number of total and apoptotic cells in blastocyst, and expression of Bax, Bcl-2, and p53 genes in blastocyst cells were determined. Compared with the control, no improvement was observed in cumulus expansion in ALA-treated groups. At 50 μM concentration, ALA increased meiotic maturation rate but had no effect on GSH level. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher rate of blastocyst formation was observed, and these embryos had a higher total cell number and a lower apoptotic cell number. Expression analyses of genes in blastocysts revealed lesser transcript abundances for Bax gene, and higher transcript abundances for Bcl-2 gene in 50 μM ALA group. Expression of p53 gene was also less observed in ALA-treated blastocysts. Our results show that ALA treatment at 50 μM during in vitro maturation (IVM) had a beneficial effect on maturation of goat oocytes and this, in turn, stimulated embryonic development and regulated apoptotic gene expression.
DOI：
10.1073/pnas.180295197
发表时间：
2000-08-29
影响因子：
11.1
作者：
Elvin, JA;Yan, CN;Matzuk, MM
通讯作者：
Matzuk, MM
影响因子：
1.7
作者：
Bernal Ulloa, Sandra Milena;Heinzmann, Julia;Niemann, Heiner
通讯作者：
Niemann, Heiner