Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2
Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2
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DOI：
10.1016/j.watres.2005.12.047
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发表时间：
2006-03-01
期刊：
影响因子：
12.8
通讯作者：
Shimizu, N
中科院分区：
文献类型：
--
作者：
Dadjour, MF;Ogino, C;Shimizu, N
超声波处理系统，使用二氧化钛光催化剂，被用来消毒嗜肺军团菌。该过程的动力学研究表明，TiO 2显着改善消毒过程。在存在0.2 g/ml TiO 2的情况下，30 min的处理期后，活细胞的浓度降低至初始浓度的6%，而在不存在TiO 2的情况下仅观察到18%的降低。在一定程度上，通过增加TiO 2的使用量，可以提高消毒的效力。在1.0 g/ml TiO 2存在下，细胞浓度在治疗30 min内降低了3个数量级。还比较了TiO 2与Al 2 O3存在下的消毒能力，发现TiO 2诱导更高的细胞杀伤。在10（2）-10（7）CFU/ml的范围内，在30 min的处理期后，未发现初始细胞浓度对消毒的显著影响。通过研究OH自由基清除剂如抗坏血酸、组氨酸和谷胱甘肽的作用，研究了细胞杀伤机制。在含有这些自由基清除剂的样品中，消毒能力降低，从而表明OH自由基的重要性。(c)2006爱思唯尔有限公司版权所有。
An ultrasonic treatment system, using a TiO2 photocatalyst, was used to disinfect Legionella pneumophila. A kinetic study of the process indicates that TiO2 Significantly improves the disinfection process. The concentrations of viable cells were reduced to 6% of the initial concentrations in the presence of 0.2 g/ml TiO2 after a 30 min of treatment period, while only an 18% reduction was observed in the absence of TiO2. The potency of the disinfection could be enhanced, to some extent, by increasing the amount of TiO2 used. Cell concentrations were decreased by an order of 3 within 30 min of treatment in the presence of 1.0 g/ml TiO2. The disinfection power in the presence of TiO2 versus Al2O3 was also compared and the findings showed that TiO2 induced a higher cell killing. No significant effect of initial cell concentration on the disinfection was found in the range of 10(2)-10(7) CFU/ml after a 30 min of treatment period. The mechanism of cell killing was investigated by examining the effects of OH radical scavengers such as ascorbic acid, histidine and glutathione. The disinfection power was reduced in samples that contained these radical scavengers, thus indicating the importance of OH radicals. (c) 2006 Elsevier Ltd. All rights reserved.