Three new species of soil-inhabiting Trichoderma from southwest China
Three new species of soil-inhabiting Trichoderma from southwest China
复制标题
中国西南土壤木霉属三个新种
DOI：
10.3897/mycokeys.44.30295
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发表时间：
2018-12-14
期刊：
影响因子：
3.3
通讯作者：
Yu, ZeFen
中科院分区：
文献类型：
--
作者：
Qiao, Min;Du, Xing;Yu, ZeFen
摘要木霉属真菌在中国地区分布广泛，云南省也有分布。在本研究中，我们报道了木霉中三个新的土壤栖息种，命名为库明氏木霉、特殊木霉和泽罗哈茨木霉。描述了它们的菌落和菌丝形态，包括无性状态的特征。每个物种的DNA序列都是从核糖体DNA的内转录间隔区(ITS)、翻译延伸因子1-α编码基因(TEF1)和编码第二大核RNA聚合酶亚基(RPB2)的基因中获得的。我们的分析表明，这三个新物种在彼此之间以及与其他已知和密切相关的物种之间显示出一致的差异。在这三个种中，特殊毛霉和昆明根毛虫属于绿色枝状菌。具体地说，它与三个种有关--Hispanicum、T.samuelsii和T.jii，其特征是分生孢子柄树状，一般成对分枝，弯曲的顶生分枝，细长到纺锤形的纤毛虫和近球形到球状的分生孢子。Kunmingense在形态上与T.asperellum和T.yunnanense相似，其特征是分生孢子为金字塔形，壶状至锥形，分枝离散，分生孢子卵形，偶有椭圆形，壁光滑。第三个新种，T.zeloharzianum，是Harzianum Clade的一个新成员，与T.harzianum，T.lixii和T.simmonsii关系密切，但与它们的区别是分生孢子较小，近球形到球形，薄壁。
Abstract Fungi in the genus Trichoderma are widely distributed in China, including in Yunnan province. In this study, we report three new soil-inhabiting species in Trichoderma, named as T.kunmingense, T.speciosum and T.zeloharzianum. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the translation elongation factor 1-α encoding gene (tef1) and the gene encoding the second largest nuclear RNA polymerase subunit (rpb2). Our analyses indicated that the three new species showed consistent divergence amongst each other and from other known and closely related species. Amongst the three, T.speciosum and T.kunmingense belong to the Viride Clade. Specifically, T.speciosum is related to three species – T.hispanicum, T.samuelsii and T.junci and is characterised by tree-like conidiophores, generally paired branches, curved terminal branches, spindly to fusiform phialides and subglobose to globose conidia. In contrast, T.kunmingense morphologically resembles T.asperellum and T.yunnanense and is distinguished by its pyramidal conidiophores, ampulliform to tapered phialides, discrete branches and ovoidal, occasionally ellipsoid, smooth-walled conidia. The third new species, T.zeloharzianum, is a new member of the Harzianum Clade and is closely associated with T.harzianum, T.lixii and T.simmonsii but distinguished from them by having smaller, subglobose to globose, thin-walled conidia.