Whole Mitochondrial Genome Sequencing and Transcriptional Analysis to Uncover an RT102-Type Cytoplasmic Male Sterility-Associated Candidate Gene Derived from Oryza rufipogon
Whole Mitochondrial Genome Sequencing and Transcriptional Analysis to Uncover an RT102-Type Cytoplasmic Male Sterility-Associated Candidate Gene Derived from Oryza rufipogon
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DOI：
10.1093/pcp/pct102
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发表时间：
2013-09-01
影响因子：
4.9
通讯作者：
Toriyama, Kinya
中科院分区：
文献类型：
--
作者：
Okazaki, Masayuki;Kazama, Tomohiko;Toriyama, Kinya
细胞质雄性不育（CMS）是一种母系遗传的性状，其中植物不能产生功能性花粉，并且与由线粒体基因组编码的新的开放阅读框架（orf）基因的表达相关。以普通野生稻W1125和普通野生稻O. rufipogon W1125为材料，通过连续回交获得了RT 102 A不育系和RT 102 C恢复系。65.第六十五章使用下一代焦磷酸测序，我们确定了RT 102-CMS细胞质中线粒体的全基因组序列。为了鉴定RT 102线粒体中CMS相关基因的候选者，我们筛选了线粒体基因组中是否存在嵌合的或其产物携带预测的跨膜结构域的特定ORF基因。这些orf基因，orf 352，这表明不同的转录本大小取决于是否育性恢复（Rf）基因的存在与否，被确定。orf 352基因与核糖体蛋白基因rpl 5共转录，并且2.8kb rpl 5-orf 352转录物在Rf基因存在下加工成在orf 352编码区内部具有切割的2.6kb转录物。orf 352基因是RT 102-CMS的CMS相关基因的一个很好的候选基因。
Cytoplasmic male sterility (CMS) is a maternally inherited trait in which plants fail to produce functional pollen and is associated with the expression of a novel open reading frame (orf) gene encoded by the mitochondrial genome. An RT102A CMS line and an RT102C fertility restorer line were obtained by successive backcrossing between Oryza rufipogon W1125 and O. sativa Taichung 65. Using next-generation pyrosequencing, we determined whole-genome sequences of the mitochondria in RT102-CMS cytoplasm. To identify candidates for the CMS-associated gene in RT102 mitochondria, we screened the mitochondrial genome for the presence of specific orf genes that were chimeric or whose products carried predicted transmembrane domains. One of these orf genes, orf352, which showed different transcript sizes depending on whether the restorer of fertility (Rf) gene was present or not, was identified. The orf352 gene was co-transcribed with the ribosomal protein gene rpl5, and the 2.8 kb rpl5-orf352 transcripts were processed into 2.6 kb transcripts with a cleavage at the inside of the orf352 coding region in the presence of the Rf gene. The orf352 gene is an excellent candidate for the CMS-associated gene for RT102-CMS.