ESSENTIAL FUNCTION OF LINOLEIC-ACID ESTERIFIED IN ACYLGLUCOSYLCERAMIDE AND ACYLCERAMIDE IN MAINTAINING THE EPIDERMAL WATER PERMEABILITY BARRIER - EVIDENCE FROM FEEDING STUDIES WITH OLEATE, LINOLEATE, ARACHIDONATE, COLUMBINATE AND ALPHA-LINOLENATE
ESSENTIAL FUNCTION OF LINOLEIC-ACID ESTERIFIED IN ACYLGLUCOSYLCERAMIDE AND ACYLCERAMIDE IN MAINTAINING THE EPIDERMAL WATER PERMEABILITY BARRIER - EVIDENCE FROM FEEDING STUDIES WITH OLEATE, LINOLEATE, ARACHIDONATE, COLUMBINATE AND ALPHA-LINOLENATE
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DOI：
10.1016/0005-2760(85)90009-8
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
JENSEN, B
中科院分区：
文献类型：
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作者：
HANSEN, HS;JENSEN, B
必需脂肪酸缺乏的大鼠每天补充300 mg纯脂肪酸酯：油酸酯（O）、亚油酸酯（L）、花生四烯酸酯（A）和Columbinate（C），持续10天。在此期间，L组、A组和C组的大鼠均显示出其最初的高经皮水分丢失（一种典型的必需脂肪酸缺乏症症状）降低至非缺乏症大鼠（N组）中观察到的水平。O组大鼠的经皮水分丢失不受补充的影响。测定了2种表皮鞘脂的脂肪酸组成：酰基葡萄糖神经酰胺和酰基神经酰胺。低经表皮水分损失的重建可能与亚麻酸盐掺入2种表皮鞘脂有关。补充Columbinate导致在研究的表皮鞘脂中这种脂肪酸的含量相对较高。来自先前研究的合并皮肤样本的分析，其中断奶大鼠被喂食无脂肪饮食并口服补充纯α-葡萄糖。亚麻酸13周（汉森et詹森，1983）显示2种鞘脂中的多不饱和脂肪酸非常少。这些大鼠显示蒸发增加，这与必需脂肪酸缺乏大鼠相当。这些结果为亚油酸在维持表皮水渗透屏障的完整性方面的非常具体和重要的功能提供了强有力的证据。亚油酸的这种功能与其作为花生四烯酸和类花生酸前体的作用无关。
Essential fatty acid-deficient rats were supplemented with 300 mg per day of pure fatty acid esters: oleate (O), linoleate (L), arachidonate (A), and columbinate (C) for 10 days. During this period, the rats in groups L, A, and C all showed a decrease in their initially high transepidermal water loss, a classical essential fatty acid-deficiency symptom, to a level seen in non-deficient rats (group N). The transepidermal water loss in rats of group O was unaffected by the supplementation. Fatty acid composition of 2 epidermal sphingolipids, acylglucosylceramide and acylceramide, from the skin were determined. Reestablishment of a low transepidermal water loss may be associated with incorporation of linolenate into the 2 epidermal sphingolipids. Supplementation with columbinate resulted in relatively high amounts of this fatty acid in the investigated epidermal sphingolipids. Analysis of pooled skin specimens from a previous study in which weanling rats were fed a fat-free diet and supplemented orally with pure .alpha.-linolenate for 13 wk (Hansen et Jensen, 1983) revealed very little polyunsaturated fatty acid in the 2 sphingolipids. These rats showed increased evaporation which was comparable to that of essential fatty acid-deficient rats. These results give as strong evidence for a very specific and essential function of linoleic acid in maintaining the integrity of the epidermal water permeability barrier. This function of linoleate is independent of its role as precursor for arachidonate and icosanoids.