EFFECT OF PACTAMYCIN ON SYNTHESIS OF POLIOVIRUS PROTEINS - METHOD FOR GENETIC MAPPING
EFFECT OF PACTAMYCIN ON SYNTHESIS OF POLIOVIRUS PROTEINS - METHOD FOR GENETIC MAPPING
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DOI：
10.1128/jvi.8.4.395-401.1971
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发表时间：
1971-01-01
影响因子：
5.4
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
TABER, R;REKOSH, D;BALTIMORE, D
我们研究了药物帕他霉素对脊髓灰质炎病毒感染的HeLa细胞蛋白质合成的影响。在主要抑制蛋白质合成起始的浓度下，合成的脊髓灰质炎病毒蛋白质谱发生显著变化。衣壳前体NCVP 1的量相对于NCVP 2大大减少，NCVP X的量略有减少。由于人们认为脊髓灰质炎病毒基因组上只有一个主要的蛋白质合成起始位点，我们将脊髓灰质炎病毒蛋白质的这种差异效应解释为它们与起始位点的相对距离的指示。在此基础上，提出了脊髓灰质炎病毒NCVP 1、NCVP X、NCVP 2基因组（5′ → 3′）的基因顺序。
We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5′ → 3′) of NCVP 1, NCVP X, NCVP 2.