Gene expression within cell-sized lipid vesicles
Gene expression within cell-sized lipid vesicles
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DOI：
10.1002/cbic.200300630
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发表时间：
2003-11-07
期刊：
影响因子：
3.2
通讯作者：
Yoshikawa, K
中科院分区：
文献类型：
--
作者：
Nomura, S;Tsumoto, K;Yoshikawa, K
功能性蛋白质合成中观察到细胞大小的脂质囊泡封装的基因表达系统。通过荧光显微镜观察单个囊泡内rsGFP（红移绿色荧光蛋白）的表达。有趣的是，在反应的早期阶段，囊泡内的表达效率显著高于外面的溶液。在单个囊泡中合成的rsGFP是安全的，不会受到加入到外部水溶液中的蛋白酶K的攻击。对表达蛋白质的细胞大小的囊泡的研究将有助于对生命系统的某些方面的基本理解，并将有助于实际应用，如微反应器的构建。
Functional protein synthesis was observed in cell-sized lipid vesicles following encapsulation of a gene expression system. Expression of rsGFP (red-shifted green fluorescent protein) within individual vesicles was observed by fluorescence microscopy. Interestingly at the early stage of the reaction, the expression efficiency inside the vesicle was remarkable higher than that in the solution outside. The synthesized rsGFP in individual vesicles is safe from attack by proteinase K added to the external aqueous solution. Studies on cell-sized vesicles expressing protein should contribute to a fundamental understanding of certain aspects of living systems and will be useful for practical applications such as the construction of microreactors.