Identification of an envelope protein from the FRD family of human endogenous retroviruses (HERV-FRD) conferring infectivity and functional conservation among simians
Identification of an envelope protein from the FRD family of human endogenous retroviruses (HERV-FRD) conferring infectivity and functional conservation among simians
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DOI：
10.1128/jvi.78.2.1050-1054.2004
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发表时间：
2004-01-01
影响因子：
5.4
通讯作者：
Heidmann, T
中科院分区：
文献类型：
--
作者：
Blaise, S;Ruggieri, A;Heidmann, T
人内源性逆转录病毒（HERV）的HERV-W家族的成员先前已被证明编码功能性包膜，其可与人免疫缺陷病毒1型病毒粒子形成假型并赋予所得逆转录病毒颗粒感染性。在这里，我们表明，第二个包膜蛋白进行了系统的搜索融合蛋白，我们在所有的HERV编码包膜基因和属于HERV-FRD家族也可以使假型和赋予感染性。我们进一步表明，orthopathic包膜基因，从猿类，从新世界的猴子到人类，也是功能性的感染性试验中，与一个奇异的例外的gillobathic HERV-FRD基因，这是发现融合在细胞-细胞融合试验中，观察到的其他猿信封，但这是没有感染性的。FRD信封的序列比较显示了有限数量的突变之间的猿猴，和一个点突变位于TM亚基，被证明是负责的gillobacterium信封的感染性丧失。所确定的包膜的功能表征强烈指示了祖先逆转录病毒感染和内源化，其中一些包膜功能随后在进化中保留。
A member of the HERV-W family of human endogenous retroviruses (HERV) had previously been demonstrated to encode a functional envelope which can form pseudotypes with human immunodeficiency virus type 1 virions and confer infectivity on the resulting retrovirus particles. Here we show that a second envelope protein sorted out by a systematic search for fusogenic proteins that we made among all the HERV coding envelope genes and belonging to the HERV-FRD family can also make pseudotypes and confer infectivity. We further show that the orthologous envelope genes that were isolated from simians-from New World monkeys to humans-are also functional in the infectivity assay, with one singular exception for the gibbon HERV-FRD gene, which is found to be fusogenic in a cell-cell fusion assay, as observed for the other simian envelopes, but which is not infectious. Sequence comparison of the FRD envelopes revealed a limited number of mutations among simians, and one point mutation-located in the TM subunit-was shown to be responsible for the loss of infectivity of the gibbon envelope. The functional characterization of the identified envelopes is strongly indicative of an ancestral retrovirus infection and endogenization, with some of the envelope functions subsequently retained in evolution.