pH-Dependent Release of Insulin from Layer-by-Layer-Deposited Polyelectrolyte Microcapsules
pH-Dependent Release of Insulin from Layer-by-Layer-Deposited Polyelectrolyte Microcapsules
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DOI：
10.3390/polym7071269
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发表时间：
2015-07
期刊：
影响因子：
5
通讯作者：
Kentaro Yoshida;Tetsuya Ono;Yoshitomo Kashiwagi;Shigehiro Takahashi;Katsuhiko Sato;J. Anzai
中科院分区：
文献类型：
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作者：
Kentaro Yoshida;Tetsuya Ono;Yoshitomo Kashiwagi;Shigehiro Takahashi;Katsuhiko Sato;J. Anzai
将聚丙烯胺盐酸盐（PAH）和聚苯乙烯磺酸盐（PSS）、聚硫酸乙烯基（PVS）、硫酸葡聚糖（DS）等聚阴离子在含胰岛素的碳酸钙（CaCO3）微颗粒上逐层沉积制备了含胰岛素微胶囊。将CaCO3芯溶解在稀释的盐酸溶液中，得到含胰岛素的空心微胶囊。采用扫描电镜（SEM）、原子力显微镜（AFM）和ζ电位对微胶囊进行了表征。由于多环芳烃的电荷密度不同，微胶囊在pH为9.0和7.4时胰岛素的释放速度比在酸性溶液中更快。此外，当使用分子量较低的多环芳烃构建微胶囊时，胰岛素释放受到抑制，这可能是由于微胶囊的外壳较厚。结果表明含有胰岛素的微胶囊可能用于开发胰岛素输送系统。
Insulin-containing microcapsules were prepared by a layer-by-layer (LbL) deposition of poly(allylamine hydrochloride) (PAH) and polyanions, such as poly(styrenesulfonate) (PSS), poly(vinyl sulfate) (PVS), and dextran sulfate (DS) on insulin-containing calcium carbonate (CaCO3) microparticles. The CaCO3 core was dissolved in diluted HCl solution to obtain insulin-containing hollow microcapsules. The microcapsules were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM) images and ζ-potential. The release of insulin from the microcapsules was faster at pH 9.0 and 7.4 than in acidic solutions due to the different charge density of PAH. In addition, insulin release was suppressed when the microcapsules were constructed using PAH with a lower molecular weight, probably owing to a thicker shell of the microcapsules. The results suggested a potential use of the insulin-containing microcapsules for developing insulin delivery systems.