Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice
Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice
复制标题
核心蛋白聚糖促进 ORS 角质形成细胞的增殖和迁移并维持小鼠毛发生长期
DOI：
10.1111/exd.13770
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发表时间：
2018-11-01
影响因子：
3.6
通讯作者：
Lu, Zhongfa
中科院分区：
文献类型：
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作者：
Zhou, Lijuan;Jing, Jing;Lu, Zhongfa
DECORIN 是富含亮氨酸的小蛋白聚糖 (SLRP) 家族的典型成员，在许多生物过程和细胞生物途径中发挥着重要作用。我们之前表明，在毛发生长初期，小鼠背毛囊（HF）中的核心蛋白聚糖表达高度增强，而在毛发生长中期和休止期则降低，这表明核心蛋白聚糖可能调节毛囊周期和形态发生。在本研究中，为了进一步阐明DECORIN对毛细胞和循环转变的影响，采用体外过表达策略和Decorin null（Dcn（-/-））小鼠来研究DECORIN对外根鞘（ORS）角质形成细胞的影响。 DECORIN 过表达显着增强体外 ORS 角质形成细胞的增殖和迁移。此外，DECORIN 过表达上调了 WNT10b、-CATENIN 和 LEF1 的 mRNA 和蛋白表达水平。 Wnt/-连环蛋白抑制剂部分抑制了 DECORIN 过表达诱导的 ORS 角质形成细胞增殖和迁移的增加。此外，在成像和组织学分析中，与野生型小鼠相比，Dcn(-/-)小鼠的生长期缩短，β-连环蛋白表达水平较低。总而言之，这些发现表明 DECORIN 在体外促进 ORS 角质形成细胞的增殖和迁移，并维持小鼠的毛发生长期。
DECORIN is a prototypical member of the small leucine-rich proteoglycan (SLRP) family that plays important roles in numerous biological processes and cellular biological pathways. We previously showed that Decorin expression was highly enhanced in mouse dorsal hair follicles (HFs) during the anagen phase and was reduced during the catagen and telogen phases, suggesting that Decorin might modulate follicular cycling and morphogenesis. In this study, to further clarify the effects of DECORIN on hair cells and the cycling transition, an in vitro overexpression strategy and Decorin-null (Dcn(-/-)) mice were used to investigate the effects of DECORIN on outer root sheath (ORS) keratinocytes. DECORIN overexpression significantly enhanced proliferation and migration in ORS keratinocytes in vitro. Moreover, DECORIN overexpression upregulated the mRNA and protein expression levels of WNT10b, -CATENIN and LEF1. The DECORIN overexpression-induced increase in the proliferation and migration of ORS keratinocytes was partially inhibited by a Wnt/-catenin inhibitor. Furthermore, Dcn(-/-) mice had a shortened anagen phase and lower levels of -catenin expression than were observed in wild-type mice in imaging and histological analyses. Taken together, these findings suggest that DECORIN promotes the proliferation and migration of ORS keratinocytes in vitro and maintains hair anagen in mice.