Evidence for rhodopsin refolding during the decay of Meta II.
Evidence for rhodopsin refolding during the decay of Meta II.
复制标题
Meta II 衰变过程中视紫红质重折叠的证据。
DOI：
10.1016/s0006-3495(87)83341-6
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发表时间：
1987
影响因子：
3.4
通讯作者：
DeGrip,WJ
中科院分区：
文献类型：
--
作者：
Rothschild,KJ;Gillespie,J;DeGrip,WJ
傅里叶变换红外光谱 (FTIR) 显示视紫红质漂白级联的 Meta II 中间体相对于视紫红质在结构上发生了扭曲。除了之前检测到的羧基状态的改变之外，一小部分蛋白质主链经历了从 α 螺旋结构到 β 型结构的转变。所有这些变化在 Meta II 衰变期间都会部分逆转。这一证据与早期光中间体的 FTIR 研究一起表明，在已知的光中间体中，Meta II 的蛋白质结构是最扭曲的。结论是，光导致视紫红质转化为构象扭曲形式（Meta II），随后重新折叠成更像视紫红质的构象（视蛋白）。
Fourier transform infrared difference spectroscopy (FTIR) reveals that the Meta II intermediate of the rhodopsin bleaching cascade is structurally distorted relative to rhodopsin. In addition to previously detected alterations in the state of carboxyl groups, a small part of the protein back-bone undergoes a conversion from alpha-helical to beta-type structure. All of these changes partially reverse during Meta II decay. This evidence together with FTIR studies of earlier photointermediates indicates that of the known photointermediates the protein structure of Meta II is the most distorted. It is concluded that light causes rhodopsin to convert into a conformationally distorted form (Meta II), which subsequently refolds into a more rhodopsin-like conformation (opsin).