Melanosome motility in fish retinal pigment epithelial cells.
Melanosome motility in fish retinal pigment epithelial cells.
复制标题
鱼视网膜色素上皮细胞中黑素体的运动。
DOI：
10.1007/978-1-60761-376-3_15
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
King-Smith,Christina
中科院分区：
文献类型：
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作者：
King-Smith,Christina
已经开发了几种模型系统来研究细胞内细胞器运动。一个相对新颖的系统，是简单的，但用于研究细胞器运动的机制是鱼视网膜色素上皮（RPE）细胞。分离的RPE细胞的原代培养物容易制备并且适合于细胞器运动研究。在体内，RPE内的含黑色素的色素颗粒（黑素体）响应于光通量而长距离迁移。当在体外培养时，RPE细胞附着于基底，其中顶端突起从中央细胞体径向延伸。黑素体可以被化学触发以聚集或分散在整个突起中，并且可以使用相差显微镜容易地观察到。RPE顶端突起中的黑素体迁移依赖于肌动蛋白丝，因此使得该模型系统可用于研究肌动蛋白依赖的细胞器运动。
Several model systems have been developed to investigate intracellular organelle motility. A relatively novel system that is simple but useful for studying mechanisms of organelle motility is the fish retinal pigment epithelial (RPE) cell. Primary cultures of dissociated RPE cells are easily prepared and amenable to organelle motility studies. In vivo, melanin-containing pigment granules (melanosomes) within the RPE migrate long distances in response to light flux. When cultured in vitro, RPE cells attach to the substrate with the apical projections extending radially from the central cell body. Melanosomes can be chemically triggered to aggregate or disperse throughout the projections, and can be easily observed using phase contrast microscopy. Melanosome migration in RPE apical projections is dependent on actin filaments, and thus renders this model system useful for investigations of actin-dependent organelle motility.