In vitro evaluation of human endometrial stem cell-derived osteoblast-like cells' behavior on gelatin/collagen/bioglass nanofibers' scaffolds
In vitro evaluation of human endometrial stem cell-derived osteoblast-like cells' behavior on gelatin/collagen/bioglass nanofibers' scaffolds
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DOI：
10.1002/jbm.a.35748
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发表时间：
2016-09-01
影响因子：
4.9
通讯作者：
Ai, Jafar
中科院分区：
文献类型：
--
作者：
Sharifi, Esmaeel;Ebrahimi-Barough, Somayeh;Ai, Jafar
通过将含铜离子的纳米纤维生物玻璃作为骨组织的无机相以及明胶/胶原蛋白作为有机相结合，制备了一种新的仿生纳米复合支架。在本研究中，采用冷冻干燥和静电纺丝方法制备支架，并使用京尼平作为交联剂以提高支架的力学性能。在这种仿生支架上研究了人子宫内膜干细胞衍生的成骨样细胞的生长和活力。细胞生物相容性试验表明，与二维培养相比，这种支架具有更高的活力和成骨细胞生长。铜离子促进了含纳米纤维生物玻璃的纳米复合支架上成骨细胞的生长。因此，本研究结果表明，所制备的支架适合成骨细胞的生长和附着；因此，这种纳米复合支架可能是一种适用于骨组织工程的合适支架。（c）2016威利期刊公司。《生物医学材料研究A部分》：104A：2210 - 2219，2016年。
New biomimetic nanocomposite scaffold was prepared by the combination of nanofibrilar bioglass containing copper ion as the inorganic phase and gelatin/collagen as the organic phase of bone tissue. In this study for fabrication of the scaffold, freeze drying and electrospinning methods were used, and genipin was used as the cross-linking agent for increasing the mechanical properties of the scaffold. The growth and viability of human endometrial stem cell-derived osteoblast-like cells were investigated on this biomimetic scaffold. Cellular biocompatibility assays illustrated that this scaffold has more viabilities and osteoblast growths in comparison with two-dimensional culture. Copper ion increased growth of the osteoblasts on nanocomposite scaffold containing nanofibrous bioglass. Thus, the results obtained from this study indicate that the prepared scaffold is suitable for osteoblast growth and attachment; thus, potentially, this nanocomposite scaffold is an appropriate scaffold for bone tissue engineering. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2210-2219, 2016.