Thiol-based redox proteins in abscisic acid and methyl jasmonate signaling in Brassica napus guard cells.
Thiol-based redox proteins in abscisic acid and methyl jasmonate signaling in Brassica napus guard cells.
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DOI：
10.1111/tpj.12490
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Zhu M;Zhu N;Song WY;Harmon AC;Assmann SM;Chen S
可逆氧化的半胱氨酸巯基作为氧化还原传感器或氧化还原传感的目标，在不同的生理过程中是重要的。然而，人们对保卫细胞中的氧化还原敏感蛋白及其在气孔信号转导中的作用知之甚少。本研究采用互补蛋白质组学、饱和差示凝胶电泳（DIGE）和同位素编码亲和标签（ICAT）技术对脱落酸（阿坝）和茉莉酸甲酯（MeJA）诱导的油菜保卫细胞蛋白质进行了鉴定。在阿坝和MeJA处理的保卫细胞中，分别鉴定出65和118个潜在的氧化还原响应蛋白。所有的蛋白质都含有至少一个半胱氨酸，并且超过一半的蛋白质被预测形成分子内二硫键。大多数蛋白质属于能量，压力和防御以及新陈代谢的功能组。基于质谱鉴定的肽序列，阿坝和MeJA处理的样品共有30种蛋白质。在所有鉴定的蛋白质中总共绘制了44个半胱氨酸，并定量了它们的氧化还原敏感性水平。两个蛋白质，SNRK2激酶和异丙基苹果酸脱氢酶被证实是氧化还原调节和参与气孔运动。本研究建立了一个潜在的氧化还原开关清单，并强调了保卫细胞阿坝和MeJA信号转导中的蛋白质氧化还原调节机制。
Reversibly oxidized cysteine sulfhydryl groups serve as redox sensors or targets of redox sensing that are important in different physiological processes. Little is known, however, about redox sensitive proteins in guard cells and how they function in stomatal signaling. In this study, Brassica napus guard cell proteins altered by redox in response to abscisic acid (ABA) or methyl jasmonate (MeJA) were identified by complementary proteomics approaches, saturation differential in-gel electrophoresis (DIGE) and isotope-coded affinity tag (ICAT). In total, 65 and 118 potential redox responsive proteins were identified in ABA and MeJA treated guard cells, respectively. All the proteins contain at least one cysteine, and over half of them are predicted to form intra-molecular disulfide bonds. Most of the proteins fall into the functional groups of energy, stress and defense, and metabolism. Based on the peptide sequences identified by mass spectrometry, 30 proteins were common to ABA and MeJA treated samples. A total of 44 cysteines was mapped in all the identified proteins, and their levels of redox sensitivity were quantified. Two of the proteins, a SNRK2 kinase and an isopropylmalate dehydrogenase were confirmed to be redox regulated and involved in stomatal movement. This study creates an inventory of potential redox switches, and highlights a protein redox regulatory mechanism in guard cell ABA and MeJA signal transduction.
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