Functionalized surface arrays for spatial targeting of immune cell signaling
Functionalized surface arrays for spatial targeting of immune cell signaling
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DOI：
10.1021/ja058701p
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发表时间：
2006-05-03
影响因子：
15
通讯作者：
Baird, B
中科院分区：
文献类型：
--
作者：
Senaratne, W;Sengupta, P;Baird, B
表面形貌和化学对细胞反应的影响是至关重要的，特别是当生命系统遇到医疗植入物、组织工程和基于细胞的传感器等设备表面时。为了了解表面上的这些生物过程，人们对通过表面化学与高级图案化工艺相结合而产生的定制表面活性材料产生了广泛的兴趣。我们利用自组装单层（SAMs）作为亚微米尺度空间分辨率的分子模板，在大鼠嗜碱性白血病（RBL）肥大细胞上参与和聚集IgE受体。生物活性模板由硅上的金阵列组成，图案从1 μm到45 nm。这些金阵列充当分子系结位点，使功能化自组装的烷硫醇单层共价结合成为可能。单层膜的自由端被2,4-二硝基苯基(DNP)-己酸酯基配体功能化，该配体与RBL肥大细胞上与DNP高亲和细胞表面受体FcεRI结合的抗DNP IgE特异性相互作用。目前在1 μm到600 nm的结构上的研究结果表明，这些配体固定的图案阵列可以作为可视化和系统表征细胞膜参与IgE受体介导的免疫细胞信号传导的有力工具。
The effect of surface topography and chemistry on cellular response is of fundamental importance, especially where living systems encounter device surfaces as in medical implants, tissue engineering, and cell-based sensors. To understand these biological processes on surfaces, there is a widespread interest in tailored surface-active materials produced by a combination of surface chemistry coupled to advanced patterning processes. We utilize self-assembled monolayers (SAMs) as molecular templates with submicrometer-scale spatial resolution to engage and cluster IgE receptors on rat basophilic leukemia (RBL) mast cells. Bioactive templates consisted of gold arrays on silicon with patterns from 1 μm down to 45 nm. These gold arrays served as molecular tethering sites, enabling covalent binding of functionalized self-assembled monolayers of alkanethiols. The free ends of the monolayers were functionalized with 2,4-dinitrophenyl(DNP)-caproate-based ligands which interact specifically with anti-DNP IgE bound to its high affinity cell surface receptor, FcεRI on RBL mast cells. Present results on structures 1 μm down to 600 nm in size indicate that these ligand-immobilized patterned arrays can function as a powerful tool for visualization and systematic characterization of cell membrane involvement in IgE receptor-mediated immune cell signaling.