Electrospun scaffolds functionalized with heparin and vascular endothelial growth factor increase the proliferation of endothelial progenitor cells
Electrospun scaffolds functionalized with heparin and vascular endothelial growth factor increase the proliferation of endothelial progenitor cells
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DOI：
10.1088/1748-605x/aa5bbc
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发表时间：
2017-04-28
影响因子：
4
通讯作者：
Pranke, P.
中科院分区：
文献类型：
--
作者：
Braghirolli, D. I.;Helfer, V. E.;Pranke, P.
在严重的外周动脉疾病病例中，可能发生组织损失，并且可能需要使用血管移植物。然而，目前还没有合适的替代品用于小直径血管。这项工作的目的是生产具有足够性能的支架作为血管替代品的应用。通过静电纺丝技术制备聚己内酯支架。用肝素和血管内皮生长因子（VEGF）对支架表面进行功能化，并对其理化性质进行表征。将人内皮祖细胞（EPC）或间充质干细胞（MSC）接种到支架的表面上以产生内皮层。静电纺丝支架表现出与天然动脉相容的机械性能。肝素的存在防止血液在支架表面上凝固。肝素和VEGF的存在有利于MSC和EPCs在支架上的适应，与非功能化支架相关。此外，在支架上培养的EPCs保持了CD 31、CD 34和VE-钙粘蛋白基因的表达。本研究的结果表明，肝素和VEGF功能化的静电纺丝支架可以应用于血管组织工程。这些支架具有抗血栓形成的特性，有利于细胞在其表面的发展。肝素和VEGF与电纺支架的结合增加了EPC的增殖，有利于内皮层的形成和受损血管的再生。
In severe cases of peripheral arterial disease, tissue loss can occur and the use of vascular grafts can be necessary. However, currently, there are no suitable substitutes for application in small diameter vessels. The aim of this work has been to produce scaffolds with adequate properties for application as vascular substitutes. Polycaprolactone scaffolds were produced by the electrospinning technique. The surface of the scaffolds was functionalized with heparin and vascular endothelial growth factor (VEGF) and their physical-chemical properties were characterized. Human endothelial progenitor cells (EPCs) or mesenchymal stem cells (MSCs) were seeded onto the surface of the scaffolds in order to create an endothelial layer. The electrospun scaffolds exhibited mechanical properties compatible with the native arteries. The presence of heparin prevented blood coagulation on the scaffold surface. The presence of heparin and VEGF favored the adaptation of MSCs and EPCs on the scaffolds in relation to the non functionalized scaffolds. In addition, the EPCs cultivated on the scaffolds maintained the expression of CD31, CD34 and VE-cadherin genes. The results obtained in the present study suggest that electrospun scaffolds functionalized with heparin and VEGF can be applied in vascular tissue engineering. These scaffolds exhibited antithrombogenic properties and favored the development of cells on their surface. The association of heparin and VEGF with electrospun scaffolds increased EPC proliferation, favoring the formation of the endothelial layer and the regeneration of damaged vessels.