A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF SOME NEW-ZEALAND HONEYS
A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF SOME NEW-ZEALAND HONEYS
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DOI：
10.1111/j.2042-7158.1991.tb03186.x
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发表时间：
1991-12-01
影响因子：
3.3
通讯作者：
REID, GM
中科院分区：
文献类型：
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作者：
ALLEN, KL;MOLAN, PC;REID, GM
为了评估蜂蜜抗菌活性的变化，对新西兰各地商业养蜂人提供的345份未经巴氏灭菌的蜂蜜样本进行了调查。大多数蜂蜜被认为是单花的，来自26个不同的花香来源。以苯酚为标准，用琼脂扩散法检测蜂蜜对金黄色葡萄球菌的抑制作用。抗菌活性的范围从相当于2%(w/v)苯酚到58%(w/v)苯酚，中位数为13.6，标准偏差为12.5。无论是蜂蜜样本的年龄，还是它们是否经过养蜂人的处理，都与活性较低无关。然而，不同来源的花卉在抑菌活性上存在非常显著的差异。Kanuka(kunzea ericoides(A.Rich.)J·汤普森。科：桃金娘科(Myrtaceae)，木兰科(Leptosperum osperum J.R.et G.Forst.)科：桃金娘科(Myrtaceae)、灵石南属(Callunavergaris(L.)赫尔。科：杜鹃花科(Ericaceae)和总状花序豆科(Weinmannia racemosa Linn.)F.科：Cunoniaceae)可能是蜂蜜的来源，具有很高的抗菌活性。当用过氧化氢酶去除过氧化氢来测定抗菌活性时，大多数蜂蜜都没有检测到抗菌活性。只有麦卢卡和毒蛇(紫草科)蜂蜜在相当大比例的样本中显示出这种类型的活性。在许多情况下，麦卢卡蜂蜜的高抗菌活性完全是由于这种非过氧化氢成分。
To assess the variation in antibacterial activity of honey a survey was carried out on 345 samples of unpasteurized honey obtained from commercial apiarists throughout New Zealand. Most of the honeys were considered to be monofloral, from 26 different floral sources. The honeys were tested against Staphylococcus aureus in an agar well diffusion assay, with reference to phenol as a standard. Antibacterial activity was found to range from the equivalent of < 2% (w/v) phenol to 58% (w/v) phenol, with a median of 13.6 and a standard deviation of 12.5. Neither the age of the honey samples nor whether they had been processed by the apiarist was associated with lower activity. However, the difference between floral sources in the antibacterial activity was very highly significant. Kanuka (Kunzea ericoides (A. Rich.) J. Thompson. Family: Myrtaceae), manuka (Leptospermum scoparium J. R. et G. Forst. Family: Myrtaceae), ling heather (Calluna vulgaris (L.) Hull. Family: Ericaceae) and kamahi (Weinmannia racemosa Linn. f. Family: Cunoniaceae) were shown to be sources likely to give honey with high antibacterial activity. When antibacterial activity was assayed with catalase added to remove hydrogen peroxide, most of the honeys showed no detectable antibacterial activity. Only manuka and vipers bugloss (Echium vulgare L. Family: Boraginaceae) honeys showed this type of activity in a significant proportion of the samples. The high antibacterial activity of manuka honey was in many cases due entirely to this non-peroxide component.