ACCURATE INVITRO TRANSCRIPTION OF XENOPUS-LAEVIS MITOCHONDRIAL-DNA FROM 2 BIDIRECTIONAL PROMOTERS
ACCURATE INVITRO TRANSCRIPTION OF XENOPUS-LAEVIS MITOCHONDRIAL-DNA FROM 2 BIDIRECTIONAL PROMOTERS
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DOI：
10.1128/mcb.6.7.2543
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发表时间：
1986-07-01
影响因子：
5.3
通讯作者：
YOZA, BK
中科院分区：
文献类型：
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作者：
BOGENHAGEN, DF;YOZA, BK
用肝素-琼脂糖凝胶层析和磷酸纤维素层析对非洲爪蟾卵母细胞线粒体RNA聚合酶进行了部分纯化。这种RNA聚合酶制剂特异性地启动了X的转录。从包含在重链复制起点和rRNA顺反子之间的mtDNA的123个碱基对区段内的两个双向启动子，体外转录起始于与体内转录起始位点完全相同的起始位点。在四个位点中的每一个，起始发生在保守的核苷酸序列ACPuTTATA内。该共有序列与人类mtDNA的转录启动子无关。
The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites previously mapped as initiation sites for transcription in vivo. At each of the four sites, initiation occurred within a conserved nucleotide sequence, ACPuTTATA. This consensus sequence is not related to promoters for transcription of human mtDNA.