ON BINDING OF CONGO RED BY AMYLOID
ON BINDING OF CONGO RED BY AMYLOID
复制标题
DOI：
10.1177/10.3.355
复制
发表时间：
1962-01-01
影响因子：
3.2
通讯作者：
LEVINE, M
中科院分区：
文献类型：
--
作者：
PUCHTLER, H;SWEAT, F;LEVINE, M
对淀粉样蛋白与刚果红结合的组织化学研究和各种磺化染料对淀粉样蛋白染色的比较表明，刚果红对淀粉样蛋白的选择性染色不能用染料的磺酸基团与淀粉样蛋白的碱性基团之间的相互作用来解释。直接棉染料的选择性染色，而不是与其他双偶氮染料，表明染料分子中的氢键基团的分布和淀粉样蛋白的亲和力之间的相关性。有人建议，淀粉样蛋白的选择性染色与刚果红是由于立体构型的淀粉样蛋白，有利于非离子结合的染料，可能是通过一种机制类似的操作中的染色纤维素材料。
Histochemical studies of the binding of Congo red by amyloid and comparison of the staining of amyloid with various sulfonated dyes demonstrate that the selective staining of amyloid with Congo red can not be explained by interaction between sulfonic acid groups of the dye and basic groups of amyloid. The selective staining with direct cotton dyes, but not with other disazo dyes, indicates correlation between the distribution of hydrogen bonding groups in the dye molecule and affinity for amyloid. It is suggested that the selective staining of amyloid with Congo red is due to steric configurations of amyloid which favor non-ionic binding of the dye, probably by a mechanism similar to that operative in the dyeing of cellulosic materials.