Enhanced expression of lncRNA TP73-AS1 predicts unfavorable prognosis for gastric cancer and promotes cell migration and invasion by induction of EMT
Enhanced expression of lncRNA TP73-AS1 predicts unfavorable prognosis for gastric cancer and promotes cell migration and invasion by induction of EMT
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DOI：
10.1016/j.gene.2018.08.055
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发表时间：
2018-12-15
期刊：
影响因子：
3.5
通讯作者：
Ma, Chunjie
中科院分区：
文献类型：
--
作者：
Zhang, Wei;Zhai, Yajuan;Ma, Chunjie
胃癌是近年来世界范围内发病率较高的恶性肿瘤。长链非编码RNA（longnoncodingRNA，lncRNA）在许多生物学过程中起重要作用，如肿瘤的发生和发展。具体来说，TP 73-AS 1是一种新型癌症相关IncRNA，据报道在多种恶性肿瘤中上调，但TP 73-AS 1在GC中的临床意义和功能作用仍不清楚。进行RTqPCR以评估GC组织标本和细胞系中TP 73-AS 1的转录。此外，TP 73-AS 1转录与临床病理特征的相关性进行了进一步评估。并在体内外观察TP 73-AS 1对胃癌细胞的作用。结果表明，TP 73-AS 1在胃癌组织和细胞中表达增强，且TP 73-AS 1的表达水平与肿瘤大小、TNM分期和总生存期密切相关。TP 73-AS 1表达降低可抑制细胞生长和集落形成能力，并通过调节Bcl-2/caspase-3通路促进细胞凋亡。重要的是，TP 73-AS 1可以促进异种移植物在体内的生长。沉默TP 73-AS 1可部分通过逆转蜗牛介导的上皮-间质转化（EMT）抑制GC细胞的迁移和侵袭特性。总的来说，这项研究可能有助于制定GC的治疗策略。
Gastric cancer (GC) is a deadly disease with high incidence worldwide in recent years. Long noncoding RNAs (lncRNAs) were found to play imperative roles in many biological processes, such as cancer development and progression. Specifically, TP73-AS1, a novel cancer-related IncRNA, was documented to be up-regulated in several malignancies, but the clinical significance and functional role of TP73-AS1 in GC is still unknown. RTqPCR was performed to evaluate TP73-AS1 transcription in GC tissue specimens and cell lines. In addition, the correlation between TP73-AS1 transcription and clinicopathologic features was further evaluated. Moreover, the effects of TP73-AS1 on GC cell were measured in vitro and in vivo. The data documented that TP73-AS1 was enhanced in GC tissues and cells, and TP73-AS1 transcription level was tightly associated with tumor size, TNM stage, and overall survival in 76 GC patients. What's more, decreased TP73-AS1 could restrain cell growth and colony-forming capacity and promoted apoptosis partly by regulating Bcl-2/caspase-3 pathway. Importantly, TP73-AS1 could promote xenograft growth in vivo. Silencing of TP73-AS1 inhibited GC cell migratory and invasive properties partly by reversing snail-mediated epithelial-to-mesenchymal transition (EMT). Collectively, this study may help to develop the treatment strategy for GC.