The normality of sperm in an infertile man with ring chromosome 15: a case report.
The normality of sperm in an infertile man with ring chromosome 15: a case report.
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具有 15 号环染色体的不育男性精子的正常性：病例报告。
DOI：
10.1007/s10815-017-1061-9
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Iwamoto T.
中科院分区：
文献类型：
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作者：
Nishikawa K;Itoi F;Nagahara M;Jose M;Matsunaga A;Ueda J;Iwamoto T.
目的分析1例环状15号染色体不育男性患者的精子染色体状态和受精能力。将1例携带15环染色体的患者的精子注射到去核小鼠卵母细胞中。结果1例携带15环染色体的患者活动精子核型正常，在1PN的染色体铺展标本中未发现15号环染色体。此外，这些活动精子还保留了受精能力。但随着精子头部长宽比的降低，受精率下降(分别为85.2%、76.2%和64.3%)，≥分别为1.5 0、1.3 0~1.4 9和1.3 0。结论精子核型正常，无环15号染色体，活动精子长径比大，具有良好的受精潜力。
PurposeThe purpose of this report is to analyze the chromosome status and fertilization capability of sperm obtained from an infertile male patient with ring chromosome 15.MethodsThis was a case report at a private in vitro fertilization clinic. A man diagnosed with severe oligozoospermia carrying ring chromosome 15. To evaluate the chromosome status and fertilization capability, sperm from a patient carrying ring chromosome 15 were injected into enucleated mouse oocytes.ResultsThe karyotypes of motile sperm from a patient carrying ring chromosome 15 were normal, and ring chromosome 15 was not observed in the chromosome spread samples of 1PN. In addition, these motile sperm retained the fertilization capability. However, the fertilization rates decreased (85.2, 76.2, and 64.3%, respectively) along with the decline of the aspect ratio of the sperm head (≥ 1.50, 1.30–1.49, and < 1.30, respectively).ConclusionsThe karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.