In-situ immun-PCR to detect antigens
In-situ immun-PCR to detect antigens
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DOI：
10.1016/s0140-6736(00)02696-9
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发表时间：
2000-09-16
期刊：
影响因子：
168.9
通讯作者：
Liu, GY
中科院分区：
文献类型：
--
作者：
Cao, Y;Kopplow, K;Liu, GY
原位免疫测定不允许检测原位PCR可获得的微小数量的靶分子。我们建立了一种高灵敏度的原位免疫PCR方法，该方法将DNA标记通过抗体-生物素-亲和素桥连接到靶分子上，并通过原位PCR进行扩增，通过杂交原位检测扩增的DNA序列。这种技术可能是唯一一种可用于检测完整细胞或组织切片中微量的生物大分子，如蛋白质，碳水化合物和脂质。
In-situ immunoassays do not allow the detection of the minute numbers of target molecules accessible with insitu PCR. We developed a highly sensitive method, termed insitu immuno- PCR, in which the DNA marker was linked to target molecules through an antibody-biotin-avidin bridge and amplified by in-situ PCR, Amplified DNA sequences were detected in situ by hybridisation. This technique may be the only one available to detect minute quantities of biological macromolecules such as proteins, carbohydrates, and lipids in intact cells or tissue sections.