Restricted mobility of cell surface proteins in the polar regions of Escherichia coli
Restricted mobility of cell surface proteins in the polar regions of Escherichia coli
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DOI：
10.1128/jb.186.9.2594-2602.2004
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发表时间：
2004-05-01
影响因子：
3.2
通讯作者：
Schwarz, H
中科院分区：
文献类型：
--
作者：
de Pedro, MA;Grünfelder, CG;Schwarz, H
大肠杆菌胞壁蛋白球囊的极区是代谢惰性的，并且随时间推移是稳定的。由于球囊和外膜紧密相连，我们研究了极性惰性胞壁蛋白是否可以限制其他细胞包膜元素的流动性。用荧光试剂共价标记细胞，在无染料培养基中追踪，并通过显微镜观察。荧光物质更有效地保留在细胞两极比在任何其他位置。高荧光强度区和低荧光强度区之间的边界相当尖锐。标记的物质主要由细胞包膜蛋白组成，其中包括Braun脂蛋白的游离形式和结合有尿苷的形式。我们的研究结果表明，至少有一些细胞包膜蛋白的流动性受到限制的区域对应的惰性胞壁蛋白的潜在领域。
The polar regions of the Escherichia coli murein sacculus are metabolically inert and stable in time. Because the sacculus and the outer membrane are tightly associated, we investigated whether polar inert murein could restrict the mobility of other cell envelope elements. Cells were covalently labeled with a fluorescent reagent, chased in dye-free medium, and observed by microscopy. Fluorescent material was more efficiently retained at the cell poles than at any other location. The boundary between high and low fluorescence intensity areas was rather sharp. Labeled material consisted mostly of cell envelope proteins, among them the free and murein-bound forms of Braun's lipoprotein. Our results indicate that the mobility of at least some cell envelope proteins is restrained at regions in correspondence with underlying areas of inert murein.