Inflammatory cytokines inhibit upregulation of glycolipid expression by Schwann cells in vitro
Inflammatory cytokines inhibit upregulation of glycolipid expression by Schwann cells in vitro
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DOI：
10.1212/wnl.51.6.1661
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发表时间：
1998-12-01
期刊：
影响因子：
9.9
通讯作者：
Skoff, A
中科院分区：
文献类型：
--
作者：
Lisak, RP;Bealmear, B;Skoff, A
目的：探讨炎症细胞产物对雪旺细胞分化和合成髓鞘糖脂的抑制作用。背景：炎症细胞浸润周围神经系统是获得性脱髓鞘神经病的特征。目前尚不清楚这些细胞在引起脱髓鞘或抑制髓鞘合成中的作用。研究方法：将非髓鞘形成的大鼠雪旺细胞与1）不同浓度的丝裂原活化的炎性细胞的活化上清液（AS）、2）8-溴环磷酸腺苷（8-bromo cyclo-adenosine monophosphate）（8 Br cAMP），已知可诱导雪旺细胞分化和糖脂合成; 3）8 Br cAMP和不同浓度的AS; 4）8 Br cAMP和阿糖胞苷（Ara Ci，其抑制许旺细胞增殖; 5）8Br cAMP、AS和Ara C;或6）另外的培养基。结果：AS抑制cAMP诱导雪旺细胞表达髓鞘相关糖脂的能力。糖脂表达的抑制是独立的能力，这些AS诱导雪旺细胞增殖。结论：这些数据表明，炎症介质能够抑制雪旺细胞分化和髓鞘合成。
Objective: To determine whether products of inflammatory cells can inhibit differentiation and synthesis of myelin glycolipids by Schwann cells. Background: Infiltration of the peripheral nervous system by inflammatory cells is a feature of acquired demyelinating neuropathies. It is not clear what role these cells have in causing demyelination or inhibiting myelin synthesis. Methods: Nonmyelinating rat Schwann cells were incubated with 1) different concentrations of activated supernatants (AS) from mitogen-activated inflammatory cells; 2) 8-bromo cyclic adenosine monophosphate (8Br cAMP), known to induce Schwann cell differentiation and synthesis of glycolipids; 3) 8Br cAMP and varying concentrations of AS; 4) 8Br cAMP and cytosine arabinoside (Ara Ci, which inhibits Schwann cell proliferation; 5) 8Br cAMP, AS, and Ara C; or 6) additional medium. Results: AS inhibits the capacity of cAMP to induce Schwann cell expression of myelin-associated glycolipids. Inhibition of glycolipid expression was independent of the capacity of these AS to induce Schwann cell proliferation. Conclusions: These data suggest that inflammatory mediators are capable of inhibition of Schwann cell differentiation and synthesis of myelin.