Systematic evaluation of sensitivity and specificity of sibship determination by using 15 STR loci.
Systematic evaluation of sensitivity and specificity of sibship determination by using 15 STR loci.
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DOI：
10.1016/j.jflm.2007.12.018
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发表时间：
2008-07-01
影响因子：
1.5
通讯作者：
Linacre, Adrian
中科院分区：
文献类型：
--
作者：
Pu, Chang En;Linacre, Adrian
亲子关系纠纷和其他形式的亲属关系测试通常使用短串联重复序列（STR）DNA位点解决。在比较两个个体的DNA图谱以确定他们是否是兄弟姐妹的情况下，会遇到同胞关系确定。如果父母中的任何一方可用于测试，则情况被简化，但是如果两个个体中的父母都不可用于DNA测试，则可以确定用于确定两个人之间的同胞关系的组合兄弟姐妹指数（CSI）。对亲属关系的支持也是基于等位基因的共享，特别是当两个等位基因在同一个基因座上共享时，称为双等位基因共享基因座（TASL）。我们报告CSI和TASL的结合，以提高同胞的决心。包括Identifiler基因座的15个STR基因座应用于三个群体，使用对全同胞或无关对。基于所获得的数据，灵敏度、特异性、阳性预测值（PPV）和阴性预测值（NPV）可应用于确定两个DNA谱是否来自全同胞对或非同胞对。本报告强调了这种形式的亲属测试固有的问题，并建议结合使用CSI和TASL的同胞关系的确定。
Paternity disputes and other forms of kinship testing are routinely resolved using short tandem repeat (STR) DNA loci. Sibship determination is encountered in instances where the DNA profiles of two individuals are compared to determine if they are siblings. If either parent is available for testing then the situation is simplified but if neither parent of the two individuals is available for DNA testing, a combined sibling indices (CSI) for the determination of sibship between two people can be determined. Support for kinship is also based upon the sharing of alleles, particularly when both alleles are shared at the same locus, termed two-allele-sharing-loci (TASL). We report on the combination of CSI and TASL to enhance the determination of sibship. The 15 STR loci that comprise the Identifiler loci were applied to three populations using pairs of full siblings or unrelated pairs. Based upon the data obtained, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) can be applied to determining whether two DNA profiles come from full or non-sibling pairs. This report highlights the problems inherent in this form of kinship testing and recommends a combination use of CSI and TASL for sibship determination.