ACTIVITY OF A MAIZE UBIQUITIN PROMOTER IN TRANSGENIC RICE
ACTIVITY OF A MAIZE UBIQUITIN PROMOTER IN TRANSGENIC RICE
复制标题
DOI：
10.1007/bf00019304
复制
发表时间：
1993-11-01
影响因子：
5.1
通讯作者：
BLECHL, AE
中科院分区：
文献类型：
--
作者：
CORNEJO, MJ;LUTH, D;BLECHL, AE
我们将玉米泛素1启动子、第一外显子和第一内含子（UBI）用于水稻（Oryza sativa L.）CV. Taipei 309）的转化实验，并研究了其在转基因愈伤组织和植株中的表达。UBI指导的瞬时基因表达水平显着高于其他启动子/内含子组合用于水稻转化。我们利用这些高水平的表达，以确定从愈伤组织衍生的原生质体与两个嵌合基因共转染获得的稳定转化体。基因由与uidA和bar标记基因的编码区融合的UBI组成（UBI：GUS和UBI：BAR）。在热胁迫下，转基因水稻原生质体和愈伤组织中UBI：GUS表达均增加。GUS活性的组织化学定位显示，UBI在快速分裂的细胞中最活跃。该启动子在许多但不是全部水稻组织中表达，并且在转基因水稻植物发育期间经历重要的活性变化。
We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants.