Chromatin looping and eRNA transcription precede the transcriptional activation of gene in the β-globin locus.
Chromatin looping and eRNA transcription precede the transcriptional activation of gene in the β-globin locus.
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DOI：
10.1042/bsr20140126
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发表时间：
2015-03-18
影响因子：
4
通讯作者：
Kim A
中科院分区：
文献类型：
--
作者：
Kim YW;Lee S;Yun J;Kim A
增强子通过染色质环与活跃转录的靶基因紧密结合。非编码 RNA 通常在活性增强子上转录，称为 eRNA（增强子 RNA）。为了探索转录激活过程中增强子-启动子环和eRNA转录的动力学，我们通过化学处理诱导β-珠蛋白基因座，并以时间过程的方式分析了β-珠蛋白基因和基因座控制区（LCR）之间的交联频率以及LCR上转录的eRNA量。 The cross-linking frequency was increased after chemical induction but before the transcriptional activation of gene in the β-globin locus. eRNA 的转录随着交联频率的增加而增加。这些结果表明染色质环化和eRNA转录先于基因的转录激活。两个事件的同时发生表明它们之间存在功能关系。增强子和启动子之间的染色质环是在化学诱导之后但在β-珠蛋白基因座中基因转录激活之前产生的。增强子 RNA 的转录随着该位点染色质环的增加而增加。
Enhancers are closely positioned with actively transcribed target genes by chromatin looping. Non-coding RNAs are often transcribed on active enhancers, referred to as eRNAs (enhancer RNAs). To explore the kinetics of enhancer–promoter looping and eRNA transcription during transcriptional activation, we induced the β-globin locus by chemical treatment and analysed cross-linking frequency between the β-globin gene and locus control region (LCR) and the amount of eRNAs transcribed on the LCR in a time course manner. The cross-linking frequency was increased after chemical induction but before the transcriptional activation of gene in the β-globin locus. Transcription of eRNAs was increased in concomitant with the increase in cross-linking frequency. These results show that chromatin looping and eRNA transcription precedes the transcriptional activation of gene. Concomitant occurrence of the two events suggests functional relationship between them. Chromatin looping between enhancer and promoter was generated after chemical induction but before the transcriptional activation of gene in the β-globin locus. Transcription of enhancer RNAs was increased in concomitant with the increase of chromatin looping in this locus.