Quantitation of fumonisin B1 and B2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection.
Quantitation of fumonisin B1 and B2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection.
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DOI：
10.1016/j.foodchem.2017.04.142
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发表时间：
2017-11
期刊：
影响因子：
8.8
通讯作者：
Lori L Smith;Kyle A Francis;Joseph T Johnson;C. Gaskill
中科院分区：
文献类型：
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作者：
Lori L Smith;Kyle A Francis;Joseph T Johnson;C. Gaskill
Pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) was determined to be effective for quantitation of fumonisins B1and B2in feed. Liquid-solid extraction, clean-up using immunoaffinity solid phase extraction chromatography, and FMOC-derivatization preceded analysis by reverse phase HPLC with fluorescence. Instrument response was unchanged in the presence of matrix, indicating no need to use matrix-matched calibrants. Furthermore, high method recoveries indicated calibrants do not need to undergo clean-up to account for analyte loss. Established method features include linear instrument response from 0.04–2.5 µg/mL and stable derivatized calibrants over 7 days. Fortified cornmeal method recoveries from 0.1–30.0 μg/g were determined for FB1(75.1%–109%) and FB2(96.0%–115.2%). Inter-assay precision ranged from 1.0%–16.7%. Method accuracy was further confirmed using certified reference material. Inter-laboratory comparison with naturally-contaminated field corn demonstrated equivalent results with conventional derivatization. These results indicate FMOC derivatization is a suitable alternative for fumonisins B1and B2quantitation in corn-based feeds.
