SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CELLULOSE - EFFECT OF BETA-D-GLUCOSIDASE ACTIVITY AND ETHANOL INHIBITION OF CELLULASES
SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CELLULOSE - EFFECT OF BETA-D-GLUCOSIDASE ACTIVITY AND ETHANOL INHIBITION OF CELLULASES
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DOI：
10.1016/0141-0229(82)90075-8
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发表时间：
1982-01-01
影响因子：
3.4
通讯作者：
MARTIN, WRB
中科院分区：
文献类型：
--
作者：
GHOSH, P;PAMMENT, NB;MARTIN, WRB
与没有酵母的糖化相比，使用木霉纤维素酶和酿酒酵母的同步糖化发酵（SSF）将纤维素水解率提高了13-30%。 SSF的最佳温度是35℃。 C.SSF中对β-D-葡糖苷酶(β-D-葡糖苷葡糖水解酶)的需求低于糖化：当β-D-葡糖苷酶的活性与滤纸活性的比率为大约时，获得最大乙醇产量。 1.0。乙醇对纤维素酶的抑制是非竞争性的，抑制常数为30.5 g l-1，但其作用不如同等浓度的纤维二糖或葡萄糖严重。没有观察到乙醇对纤维素酶[1,4-(1,3;1,4)-β-D-葡聚糖4-葡聚糖水解酶，EC 3.2.1.4]产生不可逆变性。
Compared with saccharification in the absence of yeast, simultaneous saccharification and fermentation (SSF) using Trichoderma cellulases and Saccharomyces cerevisiae enhanced cellulose hydrolysis rates by 13-30%. The optimum temperature for SSF was 35.degree. C. The requirement for .beta.-D-glucosidase (.beta.-D-glucoside glucohydrolase) in SSF was lower than for saccarification: maximal ethanol production was attained when the ratio of the activity of .beta.-D-glucosidase to filter paper activity was .apprx. 1.0. Ethanol inhibited cellulases uncompetitively, with an inhibition constant of 30.5 g l-1, but its effect was less severe than that of an equivalent concentration of cellobiose or glucose. No irreversible denaturation of cellulases [1,4-(1,3;1,4)-.beta.-D-glucan 4-glucanohydrolase, EC 3.2.1.4] by ethanol was observed.